南方农业学报2023，Vol.54Issue(12)：3591-3598,8.DOI:10.3969/j.issn.2095-1191.2023.12.013
长梗喉毛花中3种化合物抗烟草花叶病毒活性分析及其对4种防御酶活性的影响
Effects of three compounds from Comastoma pedunlulatum on the resistance to tobacco mosaic virus and activities of four defense enzymes in tobacco
摘要
Abstract
[Objective]Tobacco mosaic virus(TMV)seriously damages tobacco,potato,pepper and other food and economic crops.To provide the lead compounds and theoretical basis for the development of plant-derived pesticides that can effectively prevent and control tobacco viral diseases,the anti-TMV activities of three compounds isolated from Co-mastoma pedunlulatum and their effects on tobacco defense enzyme activities were evaluated.[Method]Nicotiana gluti-nosa L was used as the materials,there were three groups,which were compounds treatment group(swertisin,comtraide A and isoorientin),negative control(CK)group and positive control(8%ningnanmycin)group.The protective and inac-tivation effects of the compounds on TMV infection were determined by half-leaf method.Using tobacco cultivar K326 as the materials,control group including negative control(CK)and TMV-inoculated control(CK+TMV),compounds treat-ment group(swertisin,comtraide A and isoorientin)and ningnanmycin treatment group(positive control)were divided.The effects of three compounds on tobacoo defense enzymes(PPO,PAL,SOD and POD)were determined by the de-fense enzyme assay kits.Each set of experiments was repeated three times.[Result]Swertisin,comtraide A and isoorien-tin could reduce the lesion numbers of TMV on Nicotiana glutinosa plants when the tobacco leaves were first applied with compounds at 100 μg/mL and then inoculated with TMV,showing good protective effects against TMV infection,with the inhibition rates of 50.05%,57.55%and 61.73%,respectively,whereas the inhibition rate of ningnanmicin against TMV was only 47.75%.When the TMV were mixed with compounds and then inoculated the plant leaves,Swertisin and comtraide A also had good inactivation effects against TMV,whose inhibition rates were 51.65%and 45.66%,respec-tively.After application of swertisin,comtraide A and isoorientin,the maximum activities of defense enzymes PPO were reached after 24 h,12 h and 24 h,respectively;the maximum activities of PAL were reached after 72 h;the maximum activities of defense enzymes POD were reached after 24 h,48 h and 72 h,respectively.The activities of PPO,PAL and POD were significantly different from those of the control group at the maximum value,while the activities of SOD were not significantly different from those of the control group.[Conclusion]The above three compounds at the concentration of 100 μg/mL show better protective activities against TMV than the commercially available pesticide Ningnanmycin,suggesting that they have the potential to be developed into new biological pesticides or lead compound against TMV.关键词
长梗喉毛花/黄酮类/裂环环烯醚萜苷类/烟草花叶病毒/防御酶活性Key words
Comastoma pedunlulatum/flavonoids/secoiridoid glycoside/tobacco mosaic virus/defense enzyme activity分类
农业科技引用本文复制引用
程林,李卫,刘逸婷,刘真,陈永对,李干鹏..长梗喉毛花中3种化合物抗烟草花叶病毒活性分析及其对4种防御酶活性的影响[J].南方农业学报,2023,54(12):3591-3598,8.基金项目
国家自然科学基金项目(31860498,31860100) （31860498,31860100）
云南省"兴滇英才支持计划"产业创新人才项目(XDYC-CYCX-2022-0065) National Natural Science Foundation of China(31860498,31860100) （XDYC-CYCX-2022-0065）
Industrial Innovation Tal-ent Project of Yunnan Revitalization Talent Support Program(XDYC-CYCX-2022-0065) （XDYC-CYCX-2022-0065）
