南方农业学报2023，Vol.54Issue(12)：3697-3707,11.DOI:10.3969/j.issn.2095-1191.2023.12.024
归芪益母复方制剂对产后奶牛血液氧化脂质的调节作用
Regulation of compound preparation of Guiqi Yimu on oxidized lipids in blood of postpartum dairy cows
摘要
Abstract
[Objective]To investigate the regulation of oxidized lipids in the blood of postpartum dairy cows by Quiqi yimu compound preparation,with a view to further clarifying the anti-inflammatory and antioxidant mechanisms of Quiqi Yimu compound preparation,and to provide a reference basis for the application of Quiqi yimu compound preparation in the health care and disease control of postpartum dairy cows.[Method]Fifteen healthy Holstein cows with similar body condition and 2-3 parities were selected and randomly divided into the control group(group C,n=7)and the experimen-tal group(group G,n=8).The cows in the experimental group were dosed with Guiqi Yimu compound formulation 500 mL/cow after parturition for 6 consecutive d,while the cows in the control group were dosed with an equal volume of drinking water every day.Blood was collected from the tail vein before morning feeding 7 d postpartum and plasma samples were prepared.Ultra performance liquid chromatography-mass spectrometry(UPLC-MS/MS)was used to quali-tatively and quantitatively detect the content of oxidized lipids in the plasma of two groups of dairy cows,analyze the changing law of the metabolic profile of oxidized lipids in the plasma of two groups of dairy cows,and screen for the dif-ferences of oxidized lipids in the blood of postpartum dairy cows and carry out the analysis of metabolic pathway enrich-ment.[Result]A total of 44 oxidized lipids were identified in the plasma of 2 groups of cows,including 18 arachidonic acids and its metabolites,12 linolenic acids and its derivatives,9 eicosapentaenoic acids and its derivatives,4 docosa-hexaenoic acids and its derivatives,and 1 docosapentaenoic acid and its derivatives.At 7 d postpartum,plasma oxidized lipid metabolic profiles of cows in the experimental group changed significantly compared with the control group,with eight oxidized lipids significantly changed(P<0.05,VIP≥1,FC≥2,or FC≤0.5),of which five oxidized lipids molecules were significantly up-regulated,including 12-HETE,11-HETE,15-HETE,5-HETE,and 15-oxoETE,and three were significantly down-regulated,including 20-HETE,8,9-EET,and TXB2.Pathway enrichment analysis showed that the differential lipid molecules were mainly enriched in the arachidonic acid metabolic pathway,the inflammatory mediator-regulated pathway of TRP channels,and the PPAR signaling pathway.[Conclusion]Guiqi Yimu compound preparation mainly regulates lipid metabolism in postpartum cows through arachidonic acid metabolic pathway,inflammatory media-tor regulation of TRP channels,and PPAR signaling pathway,thus exerting anti-inflammatory and antioxidant effects.关键词
产后奶牛/归芪益母复方制剂/脂质组学/氧化脂质/通路富集Key words
postpartum dairy cows/Guiqi Yimu compound preparation/lipidomics/oxidized lipid/pathway enrich-ment分类
农业科技引用本文复制引用
毛永霞,王菲菲,孔维怡,岳康,王瑞玲,刘欣,丁辉,郭延生..归芪益母复方制剂对产后奶牛血液氧化脂质的调节作用[J].南方农业学报,2023,54(12):3697-3707,11.基金项目
宁夏自然科学基金项目(2023AAC03103) Ningxia Natural Science Foundation(2023AAC03103) （2023AAC03103）
