南方农业学报2023，Vol.54Issue(12)：3708-3718,11.DOI:10.3969/j.issn.2095-1191.2023.12.025
牦牛MPC1和MPC2基因克隆及其组织表达特征分析
Cloning and tissue expression characteristics of MPC1 and MPC2 genes in Bos grunniens
摘要
Abstract
[Objective]To clarify the molecular biological and tissue expression characteristics of mitochondrial pyru-vate carrier encoding genes(MPC1 and MPC2)in yak(Bos grunniens),and provide basic data for further research on the mechanism of MPC genes in the plateau adaptation and energy metabolism of yak.[Method]Maiwa yak was selected as the research object,the coding region(CDS)sequence of MPC1 and MPC2 genes were cloned by PCR.The protein struc-ture and physicochemical properties were predicted by online bioinformatics analysis software,and their expression levels in different tissues of different ages yak(0.5-year-old,1.5-year-old and 2.5-year-old)were detected by real-time fluores-cence quantitative PCR.[Result]The length of the CDS sequence obtained by cloning the yak MPC1 gene was 330 bp,and the number of encoded amino acids residues was 109,the length of CDS region of MPC2 gene was 384 bp,and the number of encoded amino acids was 127.Phylogenetic tree based on CDS sequence similarity of MPC1 and MPC2 genes showed that yak was most closely related to Bos taurus and Bubalus bubalis respectively,and most far from Oris aries.MPC1 and MPC2 proteins were stable hydrophilic proteins,which dominated by alanine and leucine,which had trans-membrane structure and no signal peptide,had one MPC domain.The secondary structure was dominated by α-helix(57.80%and 55.12%),and the model of the tertiary structure was 5xpd.1.A.Among the 0.5-year-old,1.5-year-old and 2.5-year-old the expression levels of MPC1 and MPC2 genes were the lowest in the spleen of yak,followed by spleen and liver,and the highest in the heart.However,the expression patterns of MPC1 and MPC2 genes in different tissues of yak at three ages were inconsistent that may be related to the tissue specificity of gene expression and their different roles in different growth and development stages of yak.[Conclusion]The MPC1 and MPC2 genes exhibit high conservation across ruminants,with their expression being both tissue-specific and sequential,particularly in the heart.The encoded protein is notably abundant in alanine and leucine,suggesting a crucial role of the MPC1 and MPC2 genes in meat quality and plateau adaptation,but their functions vary during different growth stages of yak.关键词
牦牛/线粒体丙酮酸载体(MPC)/MPC1基因/MPC2基因/组织表达特征/高原环境Key words
yak(Bos grunniens)/mitochondrial pyruvate carrier(MPC)/MPC1 gene/MPC2 gene/tissue expres-sion characteristics/plateau environment分类
农业科技引用本文复制引用
姜辉,黄慈,钟金城,鲜莉莉,信金伟,王吉坤..牦牛MPC1和MPC2基因克隆及其组织表达特征分析[J].南方农业学报,2023,54(12):3708-3718,11.基金项目
西藏重大科技专项(XZ202101ZD0002N-01) （XZ202101ZD0002N-01）
省部共建青稞和牦牛种质资源与遗传改良国家重点实验室自主研发项目(XZNKY-2021-C-014-Z03) （XZNKY-2021-C-014-Z03）
第二次青藏高原综合科学考察研究项目(2019QZKK05010705) （2019QZKK05010705）
国家现代农业产业技术体系建设专项(CARS-37) Xizang Key Science and Technology Project(XZ202101ZD0002N-01) （CARS-37）
Project of State Key Laboratory of Highland Barley and Yak Germplasm Resources and Genetic Improvement(XZNKY-2021-C-014-Z03) （XZNKY-2021-C-014-Z03）
Second Qinghai-Xizang Plateau Scientific Expedition and Research Project(2019QZKK05010705) （2019QZKK05010705）
China Agriculture Re-search System(CARS-37) （CARS-37）
