华南农业大学学报2024，Vol.45Issue(1)：15-22,8.DOI:10.7671/j.issn.1001-411X.202209038
甘露寡糖干预采食高脂饲粮罗曼蛋鸡的脂肪肝综合征
Intervention of mannan oligosaccharide on fatty liver syndrome in Roman laying hens fed high-fat diet
摘要
Abstract
[Objective]The purpose of this study was to investigate the effect of dietary supplementation of mannose oligosaccharide(MOS)on fatty liver syndrome and intestinal flora of Roman laying hens.[Method]Eighty 230-day-old Roman laying hens were divided into four groups with 20 hens in each group,including the normal diet group(CK group),high-fat diet group(HFD group),HFD+0.1％MOS group(0.1％MOS group),and HFD+0.5％MOS group(0.5％MOS group).Samples of serum,liver,abdominal fat and intestinal contents were taken at the 9th week of the experiment.The regulatory effect of MOS on fatty liver in Roman hens was investigated by analyzing the serum indicators and gene expression in liver and gut microbiota sequencing.[Result]High-fat diet significantly increased body weight,liver weight,liver index,abdominal fat weight and abdominal fat index of Roman laying hens,and induced fatty liver in laying hens.MOS significantly reduced the increase of body weight,liver weight and abdominal fat weight of laying hens caused by HFD.MOS significantly inhibited the increase of fat deposition in the serum and liver of laying hens caused by HFD.MOS inhibited mRNA expression levels of fat synthesis genes ACC,FAS and SCREBP-1c in liver,and alleviated fatty liver of laying hens.MOS significantly increased the relative abundance of Bacteroidetes and decreased the relative abundance of Firmicutes in cecum of laying hens.[Conclusion]Dietary supplementation of MOS in HFD can improve the gut microbiome of laying hens,and inhibit mRNA expression levels of genes related to fat synthesis in liver,thereby alleviating the fatty liver of laying hens caused by HFD.Addition of 0.5％MOS in diet has a better effect for reducing the body weight,liver weight and abdominal fat weight of laying hens.关键词
甘露寡糖/高脂日粮/罗曼蛋鸡/脂肪肝综合征/肠道菌群Key words
Mannose oligosaccharide/High-fat diet/Roman laying hen/Fatty liver syndrome/Intestinal flora分类
农业科技引用本文复制引用
赵伟杰,江青艳,冯晓华,梁競文,莫颖芬,朱灿俊,束刚,高萍,王松波,王丽娜..甘露寡糖干预采食高脂饲粮罗曼蛋鸡的脂肪肝综合征[J].华南农业大学学报,2024,45(1):15-22,8.基金项目
国家自然科学基金(31790411) （31790411）
