鸡circSFMBT2克隆及对DF-1细胞增殖的影响OA北大核心CSTPCD
Cloning of chicken circSFMBT2 and its effect on proliferation of DF-1 cells
[目的]验证鸡circSFMBT2的环形结构,探究其表达规律及功能.[方法]以麒麟鸡和DF-1细胞为研究对象,根据反向剪切位点序列特征设计引物,通过PCR和测序验证circSFMBT2的环形结构.通过RNase R和反转录试验分析circSFMBT2的基本特性,利用qRT-PCR探究不同组织、不同时期circSFMBT2的表达水平.通过构建过表达载体,结合qRT-PCR、Edu和CCK-8等试验,探究circSFMBT2对鸡DF-1细胞增殖的影响…查看全部>>
[Objective]In order to verify the circular structure of chicken circSFMBT2 and explore its expression and functions.[Method]Kirin chicken and DF-1 cells were used in this study.Primers were designed according to the reverse splicing site,and the circular structure of circSFMBT2 was verified by PCR and sequencing.The characteristics of circSFMBT2 were analyzed by RNase R and reverse transcription experiments.The expression levels of circSFMBT2 in different ti…查看全部>>
焦振海;王府建;张丽;李国;宾乘锋;张为露;郭东雪;王晓彤;林树带
广东海洋大学滨海农业学院,广东湛江 524088广东海洋大学滨海农业学院,广东湛江 524088广东海洋大学滨海农业学院,广东湛江 524088广东海洋大学滨海农业学院,广东湛江 524088广东海洋大学滨海农业学院,广东湛江 524088广东海洋大学滨海农业学院,广东湛江 524088广东海洋大学滨海农业学院,广东湛江 524088广东海洋大学滨海农业学院,广东湛江 524088广东海洋大学滨海农业学院,广东湛江 524088
畜牧业
鸡SFMBT2环状RNADF-1细胞细胞增殖
ChickenSFMBT2circRNADF-1 cellCell proliferation
《华南农业大学学报》 2024 (1)
鸡生长激素受体基因环状RNA调控肌肉发育的作用机制研究
23-30,8
国家自然科学基金(31972550)广东省自然科学基金(2020B1515420008)
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