临床口腔医学杂志2024，Vol.40Issue(3)：136-140,5.DOI:10.3969/j.issn.1003-1634.2024.03.003
探究焦亡与原发性干燥综合征的关系
Study on the relationship between pyroptosis and primary Sjögren's syndrome
陆晓翠 1侯辰兴 1程波1
作者信息
- 1. 武汉大学中南医院口腔科 湖北 武汉 430071
- 折叠
摘要
Abstract
Objective:To explore the relationship between pyroptosis and primary Sjögren's syndrome.Methods:A253 cell line was treated with IFN-γ.Cell viability was detected by CCK-8 assay and cell viability staining.mRNA levels of pyroptosis-related markers were measured by quantitative real-time PCR(qRT-PCR).Protein levels of pyroptosis-related markers were detected by immunofluorescence.Immunohistochemistry was performed to detect the protein levels of pyroptosis-related markers in human tissue samples.A253 cells treated with IFN-γ and DMSO were separately treated with the pyropto-sis inhibitor Vx765.Cell viability and mRNA levels of related factors were detected in corresponding groups.Results:The via-bility of cells in the IFN-γ treatment group decreased significantly.mRNA and protein levels of pyroptosis-related markers in-creased.The expression of pyroptosis-related pathway proteins in human tissue samples also increased.Furthermore,after treatment with Vx765,the viability of cells in the IFN-γ group increased,and mRNA levels of pyroptosis-related inflammatory factors decreased.Conclusion:Primary Sjögren's syndrome may be accompanied by pyroptosis during its occurrence.关键词
焦亡/原发性干燥综合征/炎症Key words
Pyroptosis/Primary Sjögren's syndrome/Inflammation分类
医药卫生引用本文复制引用
陆晓翠,侯辰兴,程波..探究焦亡与原发性干燥综合征的关系[J].临床口腔医学杂志,2024,40(3):136-140,5.
