农业生物技术学报2024，Vol.32Issue(4)：859-872,14.DOI:10.3969/j.issn.1674-7968.2024.04.011
贝莱斯芽胞杆菌YFB3-1对辣椒疫霉的抑菌效果及其抑菌物质
Antibacterial Effect of Bacillus velezensis YFB3-1 on Phytophthora capsici and Its Antibacterial Substances
摘要
Abstract
Pepper phytophthora blight is a soil-borne disease caused by Phytophthora capsic,which causes devastating losses of pepper(Capsicum annuum)and seriously threatens the sustainable development of pepper industry in China.In order to obtain a safe,efficient and effective biocontrol agent for the prevention of pepper phytophthora blight,a strain of Bacillus velezensis YFB3-1 isolated and identified from the intestinal bacteria of earthworms(Eisenia foetida)was selected as a biocontrol strain,and Phytophthora capsici was used as the target strain.The antibacterial effects of different types of Bacillus on P.capsici were compared by plate confrontation method,and the antibacterial effects of YFB3-1 on different soil-borne pathogenic fungi of pepper were compared,and the main antibacterial secondary metabolites and biocontrol mechanisms were analyzed.The antibacterial test showed that B.velezensis YFB3-1 had the best inhibitory effect on P.capsici,and the inhibition rate was 85.21%,which was 19.8%higher than that of B.subtilis GCK5-1.It had good antibacterial effect on different soil-borne pathogenic fungi of pepper,and had the characteristics of broad-spectrum resistance to soil-borne pathogenic fungi of pepper.Microscopic examination showed that B.velezensis YFB3-1 could lead to abnormal mycelial growth,multi-branching or disordered branches,resulting in distortion and enlargement of mycelial cells to varying degrees,and even cell wall rupture.The results of liquid chromatography tandem mass spectrometry(LC-MS)targeted metabolomics showed that the main lipopeptide compounds in the fermentation broth of B.velezensis YFB3-1 were surfactin and fengycin,which reached the highest content at 3 d of fermentation,which were 1.19×10-3 and 1.14×10-3 mg/mL,respectively.This study provides an excellent biocontrol strain for the green prevention and control of pepper blight,and provides a reference for further excavation and utilization of secondary metabolites of B.velezensis.关键词
贝莱斯芽胞杆菌/辣椒疫霉/抑菌效果/次级代谢物/脂肽类化合物Key words
Bacillus velezensis/Phytophthora capsici/Antibacterial effect/Secondary metabolite/Lipopeptide分类
农业科技引用本文复制引用
穆晓清,鲁耀雄,戴良英,黄国林,陈薇,张榉邺,韦晨曦..贝莱斯芽胞杆菌YFB3-1对辣椒疫霉的抑菌效果及其抑菌物质[J].农业生物技术学报,2024,32(4):859-872,14.基金项目
湖南省重点研发计划(2021NK2008 （）
2022NK2062) （）
湖南省自然科学基金(2020JJ4393) （2020JJ4393）
长沙市自然科学基金(kq2208105) （kq2208105）
