农业生物技术学报2024，Vol.32Issue(4)：873-881,9.DOI:10.3969/j.issn.1674-7968.2024.04.012
牦牛致病性大肠杆菌生物被膜形成能力及影响因素分析
Analysis of Biofilm Formation Ability and Influencing Factors of Yak(Bos grunniens)Pathogenic Escherichia coli
摘要
Abstract
Biofilms exist widely in the natural environment.As one of the important anti-stress mechanisms of bacteria,they have strong resistance,pathogenicity and immune escape functions.The aim of this study was to establish a biofilm-forming model strain of yak(Bos grunniens)pathogenic Escherichia coli(YPEC),and to explore the effects of various boundary factors on the biofilm formation of YPEC.In this study,the isolates strains of YPEC Tianzhu-1,Tianzhu-2,Tianzhu-3,Qinghai-2,Qinghai-3 and Qinghai-4 were selected as the research object.Microplate quantative method was used to identify the biofilm formation ability of YPEC,and strains with strong biofilm formation were selected as the dominant strains,and then the influencing factors of biofilm formation amount were analyzed.The results showed that different isolates of YPEC had different biofilm forming abilities.Tianzhu-1,Tianzhu-2,and Tianzhu-3 strains were all strong biofilm formers,while Qinghai-3 and Qinghai-4 strains were medium biofilm formers,and Qinghai-2 strain were weak biofilm formers.Among them,Tianzhu-3 strain had the strongest ability to form biofilms(P＜0.05).The optimal amount of biofilm formation of Tianzhu-3 strains was achieved when culture time was 24 h,temperature was 37℃,pH 7.5,initial bacterial solution concentration was 1.3×109 CFU/mL and 10%bacterial liquid ratio.The addition of 20 μg/mL DNA and 2%sucrose in LB medium could promote the formation of biofilm,while the addition of different concentrations of NaCl,glucose,and lactose all had inhibitory effects on the formation of biofilms.The above results provide data support and theoretical basis for YPEC prevention and control from the perspective of biofilm.关键词
牦牛致病性大肠杆菌(YPEC)/生物被膜/形成能力Key words
Yak pathogenic Escherichia coli(YPEC)/Biofilm/Formative ability分类
农业科技引用本文复制引用
高翔,黎会苹,郎啸,贺子文,王婧虹,王霞,宁建刚..牦牛致病性大肠杆菌生物被膜形成能力及影响因素分析[J].农业生物技术学报,2024,32(4):873-881,9.基金项目
甘肃省大学生就业创业能力提升工程(0602-1060201303) （0602-1060201303）
天水师范学院校列项目(CXJ2023-06) （CXJ2023-06）
