阿魏酸合成相关基因ZlALDH2C4克隆及其在菰茎部膨大发育中的响应OA北大核心CSTPCD

Ferulic acid,an important metabolic component in monolignol synthesis catalyzed by aldehyde dehydrogenase family 2 member C4(ALDH2C4),was involved in the basic defense of plant cells in response to pathogen infection,and played an important regulatory role in the formation of secondary wall and lignification differentiation in plant cells.In this research,the ALDH2C4-related gene of ZlALDH2C4(GenBank No.OQ552555)was cloned from Zizania latifolia plant with the full-length cDNA sequence of 1 527 bp,containing a ALDH2C4 domain of PLN02766.The phylogenetic tree analysis showed that ZlALDH2C4 was closely related to OsALDH2C4,a homolog protein of Oryza sativa ssp.japonica.Based on qPCR analysis,it was showed that the expression of ZlALDH2C4 was significantly up-regulated after infected by U.esculenta in stem of Z.latifolia,which had higher expression in tissues with higher degree of lignification in plants of Z.latifolia.Based on the submicroscopic observation of the different parts cells in swollen stem of normal Jiaobai and the analysis of spatial and temporal expression of ZlALDH2C4 during the swelling development of stem,it was found that the expression of ZlALDH2C4 was basically positively correlated with the changes of lignification in stem.The lower expression in the initiation and the early development stage of swollen stem was related to the lower degree of lignification,while the enhancement of lignification in swollen stem cells at the late development stage was accompanied by a significant increasing expression of ZlALDH2C4.Among the different parts of the swollen stem,the ZlALDH2C4 gene expression in the lower part with thicker secondary wall was significantly higher than that in the upper part with thinner secondary wall.The above results indicated that ZlALDH2C4 was closely related to the regulation of secondary wall formation in stem cells,and could respond to the infection induction of U.esculenta,and participate in the regulation of lignification differentiation of stem cell in Z.latifolia.This study provides a theoretical reference for the research on the regulatory mechanism of stem swelling and development induced by infection of U.esculenta.