上海海洋大学学报2024，Vol.33Issue(2)：308-316,9.DOI:10.12024/jsou.20230504188
暗纹东方鲀组织内TTX含量与钠离子通道基因表达特征分析
Analysis of tetrodotoxin content and voltage-gated sodium channel genes characteristics in the tissues of Takifugu obscurus
摘要
Abstract
In order to investigate the tetrodotoxin(TTX)content in the tissues of Takifugu obscurus and the expression characteristics of voltage gated sodium channel genes in toxic tissues,this study used enzyme-linked immunosorbent assay(ELISA)to determine the TTX content in 8 tissues,including the liver and heart of Takifugu obscurus.Real time fluorescence quantification(RT-qPCR)was used to detect the relative expression of voltage gated sodium channels α subunit gene family,including scn1aa,scn1ab,scn4aa,scn4ab,scn5aa,scn5ab,scn8aa,and scn8ab in various tissues.The results showed that the TTX content in various tissues ranged from high to low,including brain(9.521±2.816)µg/g,heart(4.271±1.129)µg/g,gills(1.586±0.527)µg/g,muscle(1.494±0.938)µg/g,liver(0.913±0.206)µg/g,skin(0.902±0.235)µg/g,intestine(0.894±0.215)µg/g,and eye(0.864±0.287)µg/g.Brain and heart tissues were weakly toxic,while liver,skin,muscle,eye,intestine,and gill tissues were slightly toxic.The RT-qPCR results showed that the relative expression levels of scn1aa,scn1ab,scn4ab,scn5aa,scn5ab,scn8aa,and scn8ab genes were the highest in liver tissue.The scn4aa gene was only expressed in muscle tissue,and only the scn4ab gene was expressed in skin tissue.Moreover,the scn4ab gene was expressed in all detected tissue types,indicating widespread expression.It is discovered that there are differences in the expression characteristics of sodium channel genes between zebrafish and Takifugu obscurus tissues.关键词
暗纹东方鲀/电压门控钠离子通道/河鲀毒素/荧光定量PCRKey words
Takifugu obscurus/voltage-gated sodium channel/tetrodotoxin/quantitative real-time PCR分类
农业科技引用本文复制引用
王婧,王润,林梦娇,王亚宁,鲍宝龙,龚小玲..暗纹东方鲀组织内TTX含量与钠离子通道基因表达特征分析[J].上海海洋大学学报,2024,33(2):308-316,9.基金项目
国家自然科学基金面上项目(31872546,32170514) （31872546,32170514）
