针刺研究2024，Vol.49Issue(3)：256-264,9.DOI:10.13702/j.1000-0607.20230515
电针对帕金森病小鼠脑肠氧化应激的影响
Effect of electroacupuncture on brain-gut oxidative stress in Parkinson's disease mice
摘要
Abstract
Objective To observe the effect of electroacupuncture(EA)on behavior,oxidative stress factors in colon and substantia nigra of Parkinson's disease(PD)mice,so as to explore the mechanism of EA in treating PD.Methods C57BL/6 mice were randomly divided into blank,model and EA groups,with 12 mice in each group.The PD mouse model was established by continuous gavage of rotenone for 4 weeks.Mice in the EA group received EA(2 Hz/15 Hz)at"Baihui"(GV20),"Quchi"(LI11)and"Zusanli"(ST36)for 20 min,5 days a week for 2 weeks.After intervention,gait analysis was used to evaluate the motor ability and motor coordination.Ink propulsion rate was used to evaluate the intestinal transport function.The level of reactive oxygen species(ROS)in the colon was detected by flow cytometry.The contents of total protein(TP),malondialdehyde(MDA)and activities of glutathione peroxidase(GSH-Px),superoxide dismutase(SOD)in colon and substantia nigra were detected by ELISA.The expression of nuclear factor E2-related factor 2(Nrf2)in substantia nigra was detected by immunofluorescence staining.Results Compared with the blank group,the average speed,step rate,normal step ratio,distance between the front and hind feet,stride length,swing speed and maximum intensity of the maximum contact area of mice in the model group were decreased(P＜0.000 1,P＜0.01,P＜0.001),the maximum change rate of gait was increased(P＜0.001)in the model group.The intestinal propulsion rate,the activities of GSH-Px and SOD in the colon and substantia nigra,and the posi-tive expression of Nrf2 in substantia nigra were decreased(P＜0.000 1,P＜0.01,P＜0.05),while the fluorescence inten-sity of ROS in the colon,the contents of MDA in colon and substantia nigra were increased(P＜0.01).Compared with the model group,the average speed,step rate,normal step ratio,distance between the front and hind feet,stride length,swing speed,and maximum intensity of the maximum contact area of the mice in the EA group were increased(P＜0.01,P＜0.05,P＜0.001,P＜0.000 1),the maximum change rate of gait was decreased(P＜0.01).The intestinal propulsion rate,the activities of GSH-Px and SOD in the colon and substantia nigra,the positive expression of Nrf2 in substantia nigra were increased(P＜0.001,P＜0.05,P＜0.000 1),while the ROS fluorescence intensity in the colon,the MDA con-tents in the colon and substantia nigra were decreased(P＜0.01).Conclusion EA can improve the movement disor-der,gait disorder and intestinal motor function of PD mice,and protect dopaminergic neurons from damage,which may be related to its effect in antagonistic brain-gut oxidative stress.关键词
帕金森病/电针/氧化应激/脑肠轴/多巴胺能神经元Key words
Parkinson's disease/Electroacupuncture/Oxidative stress/Brain-gut axis/Dopaminergic neurons引用本文复制引用
刘凡,刘智斌,马雪,王强,王渊..电针对帕金森病小鼠脑肠氧化应激的影响[J].针刺研究,2024,49(3):256-264,9.基金项目
国家自然科学基金项目(No.82204904) （No.82204904）
陕西中医药大学博士科研基金项目(No.306-17102032232) （No.306-17102032232）
陕西省教育厅科学研究计划项目(No.22JS007) （No.22JS007）
陕西省"双链融合"中青年科研创新团队项目(No.2022-SLRH-LJ-012). （No.2022-SLRH-LJ-012）
