针刺研究2024，Vol.49Issue(3)：265-273,9.DOI:10.13702/j.1000-0607.20230732
电针对糖尿病认知功能障碍大鼠PI3K/Akt/CREB信号通路和海马神经元凋亡的影响
Effects of electroacupuncture on the PI3K/Akt/CREB signaling pathway and hippocampal neuronal apoptosis in diabetic cognitive impairment rats
摘要
Abstract
Objective To observe the effects of electroacupuncture(EA)on the phosphatidylinositol-3-kinase(PI3K)/protein kinase B(Akt)/cAMP response element binding protein(CREB)signaling pathway-related proteins and hippocampal neuron apoptosis in diabetic cognitive impairment(DCI)rats,and to explore the mechanisms of EA in treating DCI.Methods Adult male SD rats were randomly divided into normal,model,and EA groups,with 12 rats in each group.The animal model of DCI was replicated using a high-fat,high-sugar diet combined with low-dose strepto-zotocin.The EA group received EA stimulation at"Yishu"(EX-B6),"Zusanli"(ST36),"Baihui"(GV20),and"Da-zhui"(GV14).Blood glucose contents of the rats in each group were measured.The Morris water maze test was used to assess the learning and memory abilities of rats.Transmission electron microscopy was used to observe the ultra-structure of hippocampal CA1 neurons.Nissl staining was used to observe the pathological changes in hippocampal CA1 neurons.TUNEL staining was used to detect the apoptosis in hippocampal CA1 neurons.Western blot was used to detect the protein expression levels of p-PI3K/PI3K and p-Akt/Akt,as well as CREB,p-CREB,cysteine aspartate pro-tease(Caspase)-3,B-cell lymphoma-2(Bcl-2),and Bcl-2 related X protein(Bax)in the hippocampal tissue of rats.Results Compared with the normal group,the rats'random blood glucose contents were significantly increased(P＜0.01),the escape latency prolonged(P＜0.01),and the original platform crossing counts reduced(P＜0.01)in the model group.Significant damage to hippocampal CA1 neurons,a significantly increased neuronal apoptosis index(P＜0.01),decreased ratio of p-PI3K/PI3K and p-Akt/Akt and expression of CREB,p-CREB and Bcl-2 proteins,increased expression of Caspase-3 and Bax proteins(P＜0.01)were observed in the hippocampal tissue of rats in the model group.Compared with the model group,the rats in the EA group showed decreased random blood glucose content(P＜0.01),shortened escape latency(P＜0.01),increased original platform crossing counts(P＜0.01),improved quantity and pathological morphology and ultrastructure of hippocampal CA1 neurons,reduced neuronal apoptosis index(P＜0.01),increased ratio of p-PI3K/PI3K and p-Akt/Akt,and expression of CREB,p-CREB and Bcl-2 proteins(P＜0.05,P＜0.01)in the hippocampal tissue,and decreased expression of Caspase-3 and Bax proteins(P＜0.01).Conclusion EA can improve the learning and memory abilities of rats with DCI,and the mechanism may be related to the regulation of the expression of PI3K/Akt/CREB signaling pathway-related proteins,which attenuates the neuronal apoptosis in the hippocampus of rats,and improves the neural function.关键词
糖尿病认知功能障碍/电针/细胞凋亡/磷脂酰肌醇-3-激酶/蛋白激酶B/cAMP反应元件结合蛋白信号通路Key words
Diabetic cognitive impairment/Electroacupuncture/Apoptosis/PI3K/Akt/CREB signaling pathway引用本文复制引用
邓倩,梁嘉琪,高晨辉,葛侠,郑紧紧,张庆萍..电针对糖尿病认知功能障碍大鼠PI3K/Akt/CREB信号通路和海马神经元凋亡的影响[J].针刺研究,2024,49(3):265-273,9.基金项目
国家自然科学基金项目(No.81574075) （No.81574075）
安徽省教育厅重点项目(No.2022AH050516) （No.2022AH050516）
杨骏全国名老中医专家传承工作室项目(No.国中医药人教发[2016]42号) （No.国中医药人教发[2016]42号）
