血小板lncRNA在肺腺癌早期筛查中的生物标志物初步研究OACSTPCD

Objective To explore the diagnostic value of platelet long non-coding RNA(lncRNA)as a biomarker for early screening of lung adenocarcinoma(LUAD).Methods The GSE183635 and GSE89843 datasets,which contained the platelet transcriptome of LUAD and healthy controls,were used for differential analysis,and the intersection of the differen-tially expressed lncRNA(DElncRNA)of the two datasets was taken.The expression levels of DElncRNA in LUAD tissues and normal control tissues were analyzed using GEPIA2.The expression levels of LINC01088 in platelets of 51 healthy con-trols and 54 LUAD patients were detected by quantitative Real-time PCR(qRT-PCR),and the diagnostic ability of each in-dex was evaluated by ROC curve.Results 8 DElncRNAs and 1 265 DElncRNAs were obtained from GSE183635 and GSE89843 datasets,respectively.The key DElncRNA LINC01088 was selected after intersection.GEPIA2 analysis showed that the expression level of LINC01088 in LUAD tissues was lower than that in normal lung tissues(P<0.05).Platelet LINC01088 was significantly downregulated in patients with LUAD and early-stage LUAD than in healthy controls(P<0.001).The area under the curve(AUC)of platelet LINC01088 in the diagnosis of LUAD was 0.755,the sensitivity was 81.1％,and the specificity was 67.9％.The AUC for early LUAD diagnosis was 0.727,the sensitivity was 80.0％,and the specificity was 67.9％.The AUC of the combined diagnostic model composed of platelet LINC01088 and carcinoembryonic antigen(CEA)for LUAD diagnosis was 0.807,the sensitivity was 89.2％,and the specificity was 71.4％.The AUC for ear-ly LUAD was 0.770,the sensitivity was 86.7％,and the specificity was 71.4％.The combined diagnostic model of platelet LINC01088 and CEA was superior to CEA in the diagnosis of LUAD and early LUAD(Z=-2.288,-2.34,both P<0.05).Conclusion LINC01088 is down-regulated in platelets of LUAD patients.Platelet LINC01088 may be a biomarker for early screening and diagnosis of LUAD.