中国输血杂志2024，Vol.37Issue(3)：325-330,6.DOI:10.13303/j.cjbt.issn.1004-549x.2024.03.011
青岛市胶州地区B(A)亚型患者的血型血清学及基因检测分析
Blood group serology and gene detection in patients with B(A)subgroup in Jiaozhou,Qingdao
秦洪伟 1王小霞 2阴瑞兰 2张树超3
作者信息
- 1. 青岛大学基础医学院免疫学系,山东青岛 266071||青岛市胶州中心医院输血科
- 2. 青岛市胶州中心医院输血科
- 3. 青岛大学基础医学院免疫学系,山东青岛 266071||青岛大学附属医院输血科
- 折叠
摘要
Abstract
Objective To investigate and analyze the serological and molecular biological characteristics of B(A)sub-group in a tertiary hospital in Jiaozhou,Qingdao.Methods From November 2019 to February 2023,the samples of 12 pa-tients were suspected to be AB subgroup by microcolumn glass bead method and saline test tube method.The exons 6 and 7 of ABO gene were further amplified,sequenced and analyzed to determine the ABO allele type.Results A total of 9 cases of B(A)subgroup were detected in26 065 patients in Jiaozhou,with a detection rate of 0.345‰(9/26 065).Among the 9 cases of B(A)subgroup,8 cases of serological reaction showed Aweak B,and the gene detection was heterozygous for BA.04 gene and O gene.One case of serological reaction showed ABweak,and the gene detection was heterozygous for BA.04 gene and A gene.Conclusion Blood group serological combined with gene detection can accurately identify ABO blood group.B(A)subgroup alleles can exist in individuals with serological reaction of ABweak.关键词
ABO血型/B(A)亚型/血型血清学/基因测序/青岛Key words
ABO blood group/B(A)subgroup/blood group serology/gene sequencing/Qingdao分类
医药卫生引用本文复制引用
秦洪伟,王小霞,阴瑞兰,张树超..青岛市胶州地区B(A)亚型患者的血型血清学及基因检测分析[J].中国输血杂志,2024,37(3):325-330,6.
