中国临床医学影像杂志2024，Vol.35Issue(3)：192-196,5.DOI:10.12117/jccmi.2024.03.009
大鼠肝纤维化、肝硬化、小肝癌MRI扩散参数与Ki-67、PCNA表达的相关性
Correlation between MRI diffusion parameters and Ki-67 and PCNA expression in rat liver fibrosis,cirrhosis and small liver cancer models
摘要
Abstract
Objective:To explore the diagnostic value of MRI diffusion weighted imaging(DWI)in rat liver fibrosis,cirrho-sis,and small liver cancer animal models,and to analyze the correlation between ADC and eADC values with cell prolifera-tion markers Ki-67 and PCNA expression.Methods:One hundred clean grade SD rats were randomly divided into two groups:the experimental group(n=85)and the control group(n=15).The experimental group received intraperitoneal injection of thioacetamide(TAA)at a fixed point,while the control group was replaced with physiological saline,300 mg/kg,3 times per week.MRI-DWI used the single shot spin echo echo planar imaging(SE-EPI)sequence,taking groups b=300s/mm2 and 600s/mm2,to generate ADC and eADC map and measure ADC and eADC values;Liver fibrosis,cirrhosis,and small liver cancer were classified according to histopathology,and correlation analysis was performed with ADC value,eADC value,and cell prolifera-tion markers Ki-67 and PCNA expression.Results:DWI parameter data of 55 rats(experimental group(n=41),control group(n= 14))was successfully obtained DWI parameter data,with a success rate of 55%(55/100).As liver fibrosis gradually progresses,DWI showed that the signal in the liver of the experimental group mice gradually increased,and high b-value images were more obvious,but the image quality was poor.The ADC value was negatively correlated with the pathological grading of liver fibrosis,cirrhosis,and small liver cancer in rats(P<0.05),while the eADC value was positively correlated(P<0.05);Ki-67 and PCNA did not significantly express in liver fibrosis and cirrhosis,but relatively more pronounced in small liver cancer.The ADC value is negatively correlated with Ki-67 and PCNA expression(P<0.05),while the eADC value is positively correlated with Ki-67 and PCNA expression(P<0.05).Conclusion:The ADC and eADC values of MRI-DWI can reflect the progressive development of liver fibrosis,cirrhosis,and small liver cancer.There is also a certain correlation between MRI-DWI parame-ters and cell proliferation markers Ki-67 and PCNA expression,which can provide a certain reference basis for clinical pre-diction of the occurrence of small liver cancer.关键词
肝纤维化/肝硬化/肝肿瘤/磁共振成像Key words
Hepatic Fibrosis/Liver Cirrhosis/Liver Neoplasms/Magnetic Resonance Imaging分类
医药卫生引用本文复制引用
王国光,孟令平,胡明秀,官兵,梁爽,洪玉萍..大鼠肝纤维化、肝硬化、小肝癌MRI扩散参数与Ki-67、PCNA表达的相关性[J].中国临床医学影像杂志,2024,35(3):192-196,5.基金项目
上海市卫生健康委员会科研课题项目(201940176). （201940176）
