中国防痨杂志2024，Vol.46Issue(4)：461-466,6.DOI:10.19982/j.issn.1000-6621.20230458
夫西地酸对分枝杆菌的体外抑菌效果评价
Evaluation of in vitro activity of fusidic acid against mycobacteria
摘要
Abstract
Objective:To evaluate the in vitro inhibitory activity of fusidic acid(FA)against mycobacteria.Methods:The standard strains and clinical isolates of mycobacteria stored in the strain bank of Beijing Chest Hospital,Capital Medical University were selected as the research strains.There were 45 standard strains of mycobacteria,including 26 rapidly growing mycobacteria(RGM)strains and 19 slowly growing mycobacteria(SGM)strains.A total of 129 clinical isolates of mycobacteria were isolated from Beijing Chest Hospital,Capital Medical University from January 2017 to December 2018,including 26 Mycobacterium tuberculosis(MTB)sensitive strains,29 multidrug-resistant(MDR)strains,30 extensive drug-resistant(XDR)strains,23 Mycobacterium avium strains and 21 Mycobacterium gordon strains.Broth microdilution method was used to test the minimal inhibitory concentrations(MIC),MIC50,MIC90 and MIC99 of mycobacteria strains against FA,and the strains with MIC≤16 μg/ml were defined as FA sensitive strains.The drug resistance of the strains to FA was analyzed.Results:The resistance rates of RGM and SGM standard strains to FA were 23.1％(6/26)and 21.1％(4/19),respectively.The MIC50,MIC90,and MIC99 values of FA for MDR-MTB clinical isolates were 4,16,and 16 μg/ml,respectively.The resistance rate of MTB clinical isolates to FA was 3.5％(3/85),with the resistance rates of MTB drug sensitive strains,MDR-MTB,and XDR-MTB strains being 0,0,and 10.0％(3/30),respectively.The resistance rates of clinical isolates of Mycobacterium avium and Mycobacterium gordon to FA were 17.4％(4/23)and 9.5％(2/21),respectively.The MIC50,MIC90,and MIC99 values of FA for clinical isolates of mycobacterium were 4,16,and＞32 μg/ml,respectively.The overall resistance rate of FA for clinical isolates of mycobacterium was 7.0％(9/129).Conclusion:FA has high antibacterial activity against mycobacterium species and has a good antibacterial effect on MTB clinical isolates.关键词
分枝杆菌属/微生物敏感性试验/抗菌药/体外研究/夫西地酸Key words
Mycobacterium/Microbial sensitivity tests/Anti-bacterial agents/In vitro/Fusidic acid分类
医药卫生引用本文复制引用
程梦丽,姜广路,霍凤敏,薛毅,于霞..夫西地酸对分枝杆菌的体外抑菌效果评价[J].中国防痨杂志,2024,46(4):461-466,6.基金项目
高层次公共卫生技术人才建设项目培养计划(学科骨干-02-49) High-level Public Health Technical Personnel Construction Project Training Plan(Discipline backbone-02-49) （学科骨干-02-49）
