中国乳业Issue(2)：46-50,5.DOI:10.12377/1671-4393.24.02.09
17味中药及其复方对奶牛乳房炎金黄色葡萄球菌的体外抑菌试验研究
Experimental Study on Bacteriostasis of 17 Chinese Herbs and Their Compounds against Staphylococcus Aureus in Cow Mastitis in Vitro
刘登堂1
作者信息
- 1. 东营市现代畜牧业发展服务中心,山东东营 257091
- 折叠
摘要
Abstract
[Objective]To explore the antibacterial effect of 17 Chinese herbs and their compounds on Staphylococcus aureus in dairy cow mastitis in vitro.[Methods]Staphylococcus aureus isolated and preserved from milk samples of cows with mastitis in a dairy farm in Shandong Province was used as the study object.Drug sensitivity test and tube double dilution method were used to determine the drug sensitivity of Staphylococcus aureus to 17 traditional Chinese medicines,and the minimum inhibitory concentration(MIC)and minimum bactericide concentration(MBC)were determined.In this study,a combination of two Chinese herbs highly sensitive to Staphylococcus aureus was selected to make a compound compound.The MIC of the compound against Staphylococcus aureus was determined by microbroth dilution checkerboard method,and the graded inhibitory concentration(FIC)of the compound was calculated.[Results]Scutellaria baicalensis,forsythia and gallnut were highly sensitive to Staphylococcus aureus.The results of MIC and MBC detection for Staphylococcus aureus were 7.813 mg/mL and 15.625 mg/mL,respectively.The results of MIC and MBC detected by forsythia and gallnut were 15.625 mg/mL and 31.25 mg/mL,respectively.The results of the detection of MIC for Staphylococcus aureus were 15.625 mg/mL for scutellaria baicalensis + forsythia,scutellaria baicalensis + gallnut and forsythia + gallnut,and there was no correlation between the two compounds.[Conclusion]The antibacterial effect of staphylococcus aureus on dairy cow mastitis may not be enhanced after the composition of single Chinese herbs.关键词
中药/奶牛乳房炎/金黄色葡萄球菌/抑菌Key words
traditional chinese medicine/cow mastitis/staphylococcus aureus/bacteriostasis引用本文复制引用
刘登堂..17味中药及其复方对奶牛乳房炎金黄色葡萄球菌的体外抑菌试验研究[J].中国乳业,2024,(2):46-50,5.
