中国乳业Issue(2)：71-75,5.DOI:10.12377/1671-4393.24.02.14
某规模化牧场致犊牛腹泻的大肠杆菌分离鉴定及耐药性检测
Isolation,Identification and Drug Resistance Detection of Escherichia coli from Calves Diarrhea in a Large-scale Dairy Farm
摘要
Abstract
[Objective]To determine the main pathogens causing diarrhea in calves and their antibiotic resistance in a large-scale dairy farm in Shihezi area,and to propose reasonable measures against calf diarrhea,providing a reference for the prevention and treatment of calf diarrhea in the farm.[Methods]This study conducted on-site epidemiological examinations,aseptically collected 15 diarrheal samples from calves,and performed pathogen isolation and purification.The pathogens causing calf diarrhea were identified through biochemical tests and molecular biology identification.Antibiotic sensitivity tests were conducted to determine the drug resistance of the pathogens.[Results]Through biochemical tests and molecular biology identification,Escherichia coli was isolated from 12 out of 15 calf diarrhea samples.The antibiotic sensitivity test results showed that the highest resistance rate of 13 E.coli strains was to streptomycin at 46.15%(6/13),followed by penicillin and ampicillin at 38.46%(5/13),with most sensitive strains being moderately or weakly sensitive;the sensitivity to tetracycline was 76.92%(10/13),and the highest sensitivity among the 13 strains was to ceftiofur and vancomycin at 84.62%(11/13),with most strains being multidrug-resistant.[Conclusion]This study provides a reference for the diagnosis and treatment of calf diarrhea in the farm by conducting pathogen isolation and identification,and antibiotic sensitivity tests on diarrheal calves.关键词
犊牛腹泻/大肠杆菌/分离鉴定/耐药性Key words
calves diarrhea/Colibacillus/separation identification/drug resistance引用本文复制引用
曹芳,魏勇,吴忧,赵鹏飞,陈钊铭,任静静,齐亚银..某规模化牧场致犊牛腹泻的大肠杆菌分离鉴定及耐药性检测[J].中国乳业,2024,(2):71-75,5.基金项目
奶牛信息智能化管理技术研究与示范(编号:NYHXGG,2023AA310) （编号:NYHXGG,2023AA310）
石河子大学高层次人才科研启动项目(编号:RCZK202042) （编号:RCZK202042）
