农学学报2024，Vol.14Issue(3)：21-27,7.
南瓜类胡萝卜素高通量提取检测体系建立及应用
High-throughput Detection System for Carotenoids:Establishment and Application in Pumpkin
摘要
Abstract
In order to establish the high-throughput system of extraction and detection for carotenoids in pumpkin,the pumpkin flesh was used as material,the optimum extraction solution,temperature and material-liquid ratio were determined and optimized by single factor experiments and L27(313)orthogonal experimental design,respectively.The results showed that ethanol was the best extraction solution for extracting carotenoids from pumpkin.The pumpkin pulp was extracted with material-ethanol liquidratio of 1:28at 55℃ for 90min,and the yield of carotenoids was significantly higher[(7.56±0.14)mg/100 g]than other combinations.Among the extraction conditions of carotenoids,temperature showed the greatest influence on extraction efficiency,followed by the interaction of material-liquid ratio×extraction time and extraction time×temperature.The carotenoid contents of 23 different types of pumpkin were detected by high-throughput extraction and detection system.The Cucurbita maximam variety of'Beili No.4'and the C.moschata×C.maximam inbred line of 20F8 showed the significantly higher carotenoid contents[(31.40±0.35)mg/100 g]and(24.72±0.72)mg/100 g,respectively)]than other tested pumpkin,which could be used for cultivating new pumpkin varieties rich in carotenoids.The efficient detection system for carotenoids content in pumpkin was labor-saving and accurate,which would improve the efficiency of pumpkin nutrition quality breeding.关键词
南瓜/类胡萝卜素/高通量测定/远缘杂交/营养品质育种Key words
pumpkin/carotenoids/high-throughput detection system/distant hybridization/nutrition quality breeding分类
农业科技引用本文复制引用
宋慧,安学君,黄芸萍,张香琴..南瓜类胡萝卜素高通量提取检测体系建立及应用[J].农学学报,2024,14(3):21-27,7.基金项目
宁波市公益类科技计划项目"类胡萝卜素与淀粉双高粉用加工型南瓜品种辅助选育与示范"(2021S016) （2021S016）
国家自然科学基金"果糖激酶对瓜类异属间嫁接亲和特性的指示作用和调控机理"(31572142). （31572142）
