ATP1B3在神经胶质瘤中表达及其对细胞增殖和迁移的影响OACSTPCD
ATP1B3 expression in gliomas and its effect on cell proliferation and migration
目的:探讨ATP1B3在神经胶质瘤细胞(U87MG)中的表达及其对细胞增殖和迁移的影响.方法:利用在线数据库CGGA及TCGA分析ATP1B3在不同级别神经胶质瘤细胞中差异表达,及其与患者生存、预后的关系;利用siRNA干扰技术瞬时敲减神经胶质瘤细胞系U87MG中ATP1B3的表达水平,通过RT-qPCR和western blot方法检测敲减效率;用CCK-8、transwell 实验检测敲减ATP1B3后神经胶质瘤细胞增殖及迁移能力变化;wes…查看全部>>
Objective:To investigate the expression of ATP1B3 in glioma and its effect on the proliferation and migration of U87MG cells.Methods:Online databases CGGA and TCGA were used to analysis the differential expression of ATP1B3 in different grades of glioma cells and its relationship with patient survival and prognosis.siRNA interference was used to transiently knock down the expression level of ATP1B3 in the glioma cell line U87MG,and the efficiency of the knoc…查看全部>>
严其康;扈清云;孙权;王彩霞;朱金玲
佳木斯大学基础医学院,黑龙江 佳木斯 154007佳木斯大学基础医学院,黑龙江 佳木斯 154007佳木斯大学基础医学院,黑龙江 佳木斯 154007佳木斯大学第一附属医院,黑龙江 佳木斯 154007佳木斯大学基础医学院,黑龙江 佳木斯 154007
临床医学
神经胶质瘤ATP1B3细胞增殖细胞迁移
GliomaATP1B3Cell proliferationCell migration
《海南医学院学报》 2024 (6)
435-441,7
This study was supported by Jiamusi University Doctoral Special Fund Project(JMSUBZ2019-04) 佳木斯大学博士专项基金项目(JMSUBZ2019-04)
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