华西口腔医学杂志2024，Vol.42Issue(2)：163-171,9.DOI:10.7518/hxkq.2024.2023245
生物钟蛋白Bmal1对实验性牙周炎相关肾损伤的影响
Impact of circadian clock protein Bmal1 on experimentally-induced periodontitis-associated renal injury
摘要
Abstract
Objective To investigate the mechanism of circadian clock protein Bmal1(Bmal1)on renal injury with chronic periodontitis,we established an experimental rat periodontitis model.Methods Twelve male Wistar rats were randomly divided into control and periodontitis groups(n=6,each group).The first maxillary molars on both sides of the upper jaw of rats with periodontitis were ligated by using orthodontic ligature wires,whereas the control group re-ceived no intervention measures.After 8 weeks,clinical periodontal parameters,including probing depth,bleeding index,and tooth mobility,were evaluated in both groups.Micro-CT scanning and three-dimensional image recon-struction were performed on the maxillary bones of the rats for the assessment of alveolar bone resorption.Histopatholo-gical observations of periodontal and renal tissues were conducted using hematoxylin-eosin(HE)and periodic acid-Schiff(PAS)staining.Renal function indicators,such as creatinine,albumin,and blood urea nitrogen levels,and oxida-tive stress markers,including superoxide dismutase,glutathione,and malondialdehyde levels,were measured using bio-chemical assay kits.MitoSOX red staining was used to detect reactive oxygen species(ROS)content in the kidneys.The gene and protein expression levels of Bmal1,nuclear factor erythroid 2-related factor 2(Nrf2),and heme oxygenase-1(HO-1)in rat renal tissues were assessed using real-time quantitative polymerase chain reaction(RT-qPCR)and immuno-histochemical staining.Results Micro-CT and HE staining results showed significant bone resorption and attachment loss in the maxillary first molar region of the periodontitis group.Histological examination through HE and PAS staining revealed substantial histopathological damage to the renal tissues of the rats in the periodontitis group.The findings of the assessment of renal function and oxidative stress markers indicated that the periodontitis group exhibited abnormal levels of oxidative stress,whereas the renal function levels showed abnormalities without statistical significance.Mito-SOX Red staining results showed that the content of ROS in the renal tissue of the periodontitis group was significantly higher than that of the control group,and RT-qPCR and immunohistochemistry results showed that the expression levels of Bmal1,Nrf2,and HO-1 in the renal tissues of the rats in the periodontitis group showed a decreasing trend.Conclu-sion Circadian clock protein Bmal1 plays an important role in the oxidative damage process involved in the renal of rats with periodontitis.关键词
牙周炎/肾损伤/昼夜节律/氧化应激/生物钟蛋白Bmal1Key words
periodontitis/renal injury/circadian rhythm/oxidative stress/circadian clock protein Bmal1分类
医药卫生引用本文复制引用
马浩楠,李琼,商雅琦,辛禧瑞,刘歆婵,武洲,于维先..生物钟蛋白Bmal1对实验性牙周炎相关肾损伤的影响[J].华西口腔医学杂志,2024,42(2):163-171,9.基金项目
Jilin Provincial Science and Technology Plan Project(20220402069GH) 吉林省科技计划项目(20220402069GH) （20220402069GH）
