华西口腔医学杂志2024，Vol.42Issue(2)：172-180,9.DOI:10.7518/hxkq.2024.2023244
表面纳米形貌对牙周膜干细胞衰老的作用研究
Effects of surface nanomorphology on the senescence of periodontal ligament stem cells
摘要
Abstract
Objective The effect of TiO2 nanotube morphology on the differentiation potency of senescent periodon-tal ligament stem cells was investigated.Methods Two types of titanium sheets with TiO2 nanotube morphology(20V-NT and 70V-NT)were prepared via anodic oxidation at 20 and 70 V separately,and their surface morphology was ob-served.Young periodontal ligament stem cells were cultivated in an osteogenic induction medium,and the most effective surface morphology in promoting osteogenic differentiation was selected.RO3306 and Nutlin-3a were used to induce the aging of young periodontal ligament stem cells,and senescent periodontal ligament stem cells were obtained.The osteo-genic differentiation of senescent periodontal ligament stem cells was induced,and the effect of surface morphology on osteogenic differentiation was observed.Results Nanotube morphology was achieved on the surfaces of titanium sheets through anodic oxidation,and the diameters of the nanotubes increased with voltage.A significant difference in the effect of nanotube morphology was found among nanotubes with different diameters in the young periodon-tal ligament stem cells.The surface nanotube morpholo-gy of 20V-NT had a more significant effect that promoted osteogenic differentiation.Compared with a smooth tita-nium sheet,the surface nanotube morphology of 20V-NT increased the number of alkaline phosphatase-positive senes-cent periodontal ligament stem cells and promoted calcium deposition and the expression of osteogenic marker genes Runt-related transcription factor 2,osteopontin,and osteocalcin.Conclusion A special nanotube morphology enhances the differentiation ability of senescent periodontal ligament stem cells,provides an effective method for periodontal re-generation,and further improves the performance of implants.关键词
表面形貌/钛/纳米管/成骨/衰老/牙周膜干细胞Key words
surface morphology/titanium/nanotubes/osteogenesis/senescence/periodontal ligament stem cells分类
医药卫生引用本文复制引用
孙艳萍,廖立..表面纳米形貌对牙周膜干细胞衰老的作用研究[J].华西口腔医学杂志,2024,42(2):172-180,9.基金项目
National Key R&D Program of China(2021YFA1100603) （2021YFA1100603）
National Nature Science Foundation of China(82071092) （82071092）
Key R&D Projects of Sichuan Province(2023YFS0056) 国家重点研发计划(2021YFA1100603) （2023YFS0056）
国家自然科学基金(82071092) （82071092）
四川省重点研发项目(2023YFS0056) （2023YFS0056）
