表面纳米形貌对牙周膜干细胞衰老的作用研究OA北大核心CSTPCDMEDLINE

Objective The effect of TiO2 nanotube morphology on the differentiation potency of senescent periodon-tal ligament stem cells was investigated.Methods Two types of titanium sheets with TiO2 nanotube morphology(20V-NT and 70V-NT)were prepared via anodic oxidation at 20 and 70 V separately,and their surface morphology was ob-served.Young periodontal ligament stem cells were cultivated in an osteogenic induction medium,and the most effective surface morphology in promoting osteogenic differentiation was selected.RO3306 and Nutlin-3a were used to induce the aging of young periodontal ligament stem cells,and senescent periodontal ligament stem cells were obtained.The osteo-genic differentiation of senescent periodontal ligament stem cells was induced,and the effect of surface morphology on osteogenic differentiation was observed.Results Nanotube morphology was achieved on the surfaces of titanium sheets through anodic oxidation,and the diameters of the nanotubes increased with voltage.A significant difference in the effect of nanotube morphology was found among nanotubes with different diameters in the young periodon-tal ligament stem cells.The surface nanotube morpholo-gy of 20V-NT had a more significant effect that promoted osteogenic differentiation.Compared with a smooth tita-nium sheet,the surface nanotube morphology of 20V-NT increased the number of alkaline phosphatase-positive senes-cent periodontal ligament stem cells and promoted calcium deposition and the expression of osteogenic marker genes Runt-related transcription factor 2,osteopontin,and osteocalcin.Conclusion A special nanotube morphology enhances the differentiation ability of senescent periodontal ligament stem cells,provides an effective method for periodontal re-generation,and further improves the performance of implants.