江西农业学报2024，Vol.36Issue(1)：42-48,7.DOI:10.19386/j.cnki.jxnyxb.2024.01.007
高温胁迫对大白菜类胡萝卜素的影响
Effect of High Temperature Stress on Carotenoids of Chinese Cabbage
摘要
Abstract
In order to investigate the response mechanism of Chinese cabbage leaf blades under high temperature stress,Chinese cabbage 245,Gailiang Qingza 3 and Ziyi with different leaf colors and stress resistance were used as experimental materials,and their seedlings were subjected to heat stress at 40℃/30℃and 60%relative humidity.The effects of stress on the growth and physiological characteristics of Chinese cabbage seedlings were researched.the results showed that the carotenoids in leaf blades of three Chinese cabbage varieties showed a trend of first decrease and then increase under heat treatment.With the increase of heat stress duration,the carotenoid content of Chinese cabbage 245 was lowest,with 123.51 μg/g at the 6th day,and then peaked to 148.64 μg/g at the 10th day.Gailiang Qingza 3 and Ziyi showed different degrees of fluctuation,and Gailiang Qingza 3 was more sensitive to heat treatment,reaching the lowest value of 97.54 μg/g at the 4th day.The carotenoid content of Ziyi showed little difference and reached the lowest level of 124.50 μg/g at the 8th day under heat treatment.Real-time fluorescence quantitative PCR results showed that BraA01g019700.3C and BraA04g000960.3C in Chinese cabbage were significantly responsive to heat stress.The transcription levels of carotenoids related genes BraA01g019700.3C and BraA04g000960.3C in Chinese cabbage increased first and then decreased.The correlation analysis results showed that the transcription abundance of BraA05g001160.3C(P＜0.01,r=0.000)and BraA01g019200.3C(P＜0.05,r=0.040)was significantly correlated with carotenoid content.关键词
高温/大白菜/类胡萝卜素/相对表达水平Key words
High temperature/Chinese cabbage/Carotenoids/Relative expression level分类
农业科技引用本文复制引用
尹莲,赵建锋,孙玉东,罗德旭,刘璐,张雪莲,白甜,顾妍,王林闯,许文钊..高温胁迫对大白菜类胡萝卜素的影响[J].江西农业学报,2024,36(1):42-48,7.基金项目
江苏省揭榜挂帅项目(JBGS[2021]073) （JBGS[2021]073）
江苏省农业科技自主创新资金项目[CX(21)2020] （21）
江苏省淮安市基础研究计划(指导性)项目(HABZ202327) （指导性）
江苏省淮安市自然科学研究计划联合专项(HABL202229). （HABL202229）
