宁夏大学学报（自然科学版）2024，Vol.45Issue(1)：60-68,9.
4株滑液囊支原体分离株P80脂蛋白的基因克隆及序列分析
Gene Cloning and Sequence Analysis of P80 Lipoprotein from Four Strains of Mycoplasma synoviae
摘要
Abstract
To investigate the function and diversity within the P80 lipoprotein family of Mycoplasma synoviae(MS),genes encoding P80-1,P80-2,and P80-3 proteins were amplified from four MS isolates using PCR and sequenced.Bioinformatics methods were applied to analyze the protein sequences of the P80 family lipopro-teins,identify their conserved structural domains and antigenic epitopes,and predict their functions.The results indicated that the P80 family lipoproteins were basic,hydrophilic,stable proteins,with a strong hydro-phobic region at the N-terminus;only the P80-2 protein had a transmembrane helix region.The positions of the signal peptides and hydrophobic regions of the P80 family lipoproteins are consistent;the three P80 proteins share 12 conserved structural domains and have 2 to 4 distinct structural domains;there are 31 to 32 antigenic epitope sites;their secondary structures are primarily composed of α-helices and random coils;and the tertiary structure predictions show that the P80-1 protein may function as an ABC transporter,the P80-2 protein as a periplasmic binding protein type Ⅱ,and the P80-3 protein is associated with cell adhesion functions.These find-ings provide a reference for further research into the function of the MS P80 family lipoproteins,as well as insights into MS pathogenic mechanisms,pathogen diagnosis,and vaccine development.关键词
滑液囊支原体/P80家族脂蛋白/基因克隆/序列分析Key words
Mycoplasma synoviae/P80 family lipoproteins/gene cloning/sequence analysis分类
生物科学引用本文复制引用
田兴苗,郭磊,王健霖,戴莎莎,司朵朵,龚振兴,李继东..4株滑液囊支原体分离株P80脂蛋白的基因克隆及序列分析[J].宁夏大学学报（自然科学版）,2024,45(1):60-68,9.基金项目
宁夏家禽疫病防控与健康养殖创新团队基金资助项目 （）
宁夏大学产教融合研究生联合培养示范基地建设基金资助项目 （）
宁夏银川市校企联合创新专项基金资助项目(2022XQ009) （2022XQ009）
