CO2浓度升高处理下宁夏枸杞糖代谢相关酶及基因表达分析OA北大核心CSTPCD
Analysis of Enzyme and Gene Expression Related to Sugar Metabolism in Lycium barbarum under Elevated CO2 Concentration Treatment
[目的]探究CO2 浓度升高对宁夏枸杞果实发育过程中碳水化合物积累的影响,为揭示枸杞果实品质形成对气候变化的响应提供参考.[方法]以宁夏枸杞'宁杞 1号'为试验材料,采用开顶气室模拟控制系统进行CO2 浓度升高处理(800±20 μmol·mol-1),以自然环境CO2 浓度(400±20 μmol·mol-1)为对照,处理 60天后收集幼果期、青果期、转色期、红果期的根、茎、叶和果实样品,测定其糖组分含量及糖代谢相关酶活性和基因表达.[结果]1)CO2 浓度升高处理可促进果实发育过程中半乳糖、蔗糖和总糖的积累,红果期半乳糖、果糖、蔗糖、总糖含量较对照分别提升 35.71%、23.18%、19.57%和 12.23%(P<0.05).2)CO2 浓度升高处理下,宁夏枸杞发育期根、茎、叶和果实组织中苹果酸合成酶(LbMS)、中性转化酶(LbAI)、蔗糖磷酸合成酶(LbSPS)及蔗糖合成酶(LbSS)活性均显著升高(P<0.05),α-半乳糖苷酶(LbGALA)活性在果实中大于叶;4个时期果实中α-半乳糖苷酶活性分别较对照显著增加 19.54%、36.68%、21.00%、24.93%,蔗糖合成酶和蔗糖磷酸合成酶在叶中的活性显著高于其他组织;果实成熟期LbGALA和LbNI基因表达量显著上调.3)CO2 浓度升高处理下,基因LbGAE、LbNI、LbSPS在茎中显著上调,LbGALA在果实中显著上调,LbMS、LbAI在根中显著上调,LbSPS、LbSS在叶中表达量显著上调.[结论]CO2 浓度升高促进宁夏枸杞糖代谢相关基因LbSPS、LbAI、LbGAL等的表达,提高酸转化酶、中性转化酶等酶活性,从而促进夏果果实中半乳糖、蔗糖和总糖的积累.
[Objective]This study aims to explore the effect of elevated CO2 concentration on carbohydrate accumulation during the development of Lycium barbarum fruits,so as to provide a reference for adaptive management of L.barbarum to cope with climate change.[Method]L.barbarum'Ningqi 1'was used as the experimental material.An open-top chamber simulation control system was applied to elevate the CO2 concentration(800±20 μmol·mol-1),with the ambient CO2 concentration(400±20 μmol·mol-1)served as the control.After 60 days of treatment,the root,stem,leaf,and fruit samples were collected respectively at the young fruit stage,green fruit stage,coloring fruit stage,and red fruit stage,and the content of their sugar components,sugar metabolism-related enzyme activity,and relevant gene expression were determined.[Result]1)The elevated CO2 concentration promoted the accumulation of lactose,sucrose,and total sugars during the fruit development,and at the red fruit stage,the content of lactose,fructose,sucrose,and total sugars significantly increased by 35.71%,23.18%,19.57%,and 12.23%,respectively,compared to the control group(P<0.05).2)Furthermore,elevated CO2 concentrations significantly(P<0.05)increased the activity of malic acid synthase(LbMS),neutral conversion enzyme(LbAI),sucrose phosphate synthase(LbSPS),and sucrose synthase(LbSS)in the root,stem,leaf,and fruit tissues of'Ningqi 1'during its development.The activity of α-galactosidase(LbGALA)increased from leaf to fruit.Under the elevated CO2 concentration treatment,the activity of the α-galactosidase in the fruits at four different stages was significantly increased by 19.54%,36.68%,21.00%,and 24.93%,respectively,compared to the control group(P<0.05).The activity of sucrose synthase and sucrose phosphate synthase in leaves was significantly higher than that in the other tissues(P<0.05).The gene expressions of LbGALA and LbNI were significantly up-regulated at the mature fruit stage(P<0.05).3)The elevated CO2 concentrations significantly up-regulated the gene expressions of LbGAE,LbNI,and LbSPS in stems,LbGALA in fruits,and LbMS and LbAI in roots.The expression levels of LbSPS and LbSS were significantly up-regulated in leaves(P<0.05).[Conclusion]The elevated CO2 concentration promotes the expression of sugar metabolism-related genes in different tissues of L.barbarum,and it also increases enzyme activity,and thereby promoting the accumulation of lactose,sucrose,and total sugars in the fruit.
冯学瑞;马亚平;刘佳欣;陆晖;李运毛;曹兵
宁夏大学葡萄酒与园艺学院 银川 750021宁夏大学林业与草业学院 银川 750021
林学
宁夏枸杞糖代谢酶活性表达量
Lycium barbarumsugar metabolismenzyme activityexpression quantity
《林业科学》 2024 (003)
大气CO2浓度升高诱导宁夏枸杞LbGALA1、LbGALA2基因参与糖代谢的调控机制
10-21 / 12
国家自然科学基金项目(32160393).
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