基于SNP分子标记的新疆野生黄花苜蓿遗传多样性分析OACSTPCD
Genetic diversity analysis of wild Medicago falcata L.in Xinjiang based on SNP molecular markers
为揭示新疆野生黄花苜蓿的遗传多样性,研究利用SLAF-seq技术对材料进行SNP开发.结果显示,材料测序平均Q30值为96.32％,平均GC值为37.99％.研究共开发了 376 641个SLAF标签,多态性SLAF标签119 331个;SNP平均完整性为24.77％,平均杂合率5.53％.供试新疆野生黄花苜蓿品系的有效等位基因数、观测等位基因数、期望杂合度、观测杂合度和Shnnon Wiener指数的平均值分别为1.380、1.594、0.220、0.117和0.327.研究表明,聚类分析将材料分为7类,PCA将材料分为3类,但样品的来源为同一个祖先.
In order to reveal the genetic diversity of wild Medicago falcata L.in Xinjiang,SLAF-seq technology was used to develop SNP materials.The results showed that the average Q30 value and GC value were 96.32％and 37.99％,respectively.A total of 376 641 SLAF tags and 119 331 polymorphic SLAF tags were developed.The average integrity of SNP was 24.77％and the average heterozygosity was 5.53％.The average values of effective allele number,observed allele number,expected heterozygosity,observed heterozygosity,and Shnnon Wiener index were 1.380,1.594,0.220,0.117,and 0.327,respectively.The study indicates that cluster analysis divides the materials into seven categories,PCA divides the materials into three categories,but the samples come from the same ancestor.
杜雨;于秀明;汪鹏;李倩;王玉祥;张博
新疆农业大学草业学院,新疆乌鲁木齐 830052
畜牧业
黄花苜蓿SLAF-seq分子标记遗传多样性群体结构分析亲缘关系分析
Medicago falcata L.SLAF-seqmolecular markersgenetic diversitypopulation structure analysisphylogenetic relationship analysis
《饲料研究》 2024 (004)
68-73 / 6
新疆维吾尔自治区重点研发任务专项计划"优质牧草高效生产与加工关键技术集成示范(项目编号:2022B02003)";新疆维吾尔自治区科技重大专项"新疆农作物种质资源征集、评价与安全保存体系构建(项目编号:2022A03004-1)";国家自然基金"新疆野生黄花苜蓿种质资源遗传特性与核心种质构建研究(项目编号:31860675)"
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