饲料研究2024，Vol.47Issue(4)：68-73,6.DOI:10.13557/j.cnki.issn1002-2813.2024.04.013
基于SNP分子标记的新疆野生黄花苜蓿遗传多样性分析
Genetic diversity analysis of wild Medicago falcata L.in Xinjiang based on SNP molecular markers
摘要
Abstract
In order to reveal the genetic diversity of wild Medicago falcata L.in Xinjiang,SLAF-seq technology was used to develop SNP materials.The results showed that the average Q30 value and GC value were 96.32％and 37.99％,respectively.A total of 376 641 SLAF tags and 119 331 polymorphic SLAF tags were developed.The average integrity of SNP was 24.77％and the average heterozygosity was 5.53％.The average values of effective allele number,observed allele number,expected heterozygosity,observed heterozygosity,and Shnnon Wiener index were 1.380,1.594,0.220,0.117,and 0.327,respectively.The study indicates that cluster analysis divides the materials into seven categories,PCA divides the materials into three categories,but the samples come from the same ancestor.关键词
黄花苜蓿/SLAF-seq/分子标记/遗传多样性/群体结构分析/亲缘关系分析Key words
Medicago falcata L./SLAF-seq/molecular markers/genetic diversity/population structure analysis/phylogenetic relationship analysis分类
农业科技引用本文复制引用
杜雨,于秀明,汪鹏,李倩,王玉祥,张博..基于SNP分子标记的新疆野生黄花苜蓿遗传多样性分析[J].饲料研究,2024,47(4):68-73,6.基金项目
新疆维吾尔自治区重点研发任务专项计划"优质牧草高效生产与加工关键技术集成示范(项目编号:2022B02003)" （项目编号:2022B02003）
新疆维吾尔自治区科技重大专项"新疆农作物种质资源征集、评价与安全保存体系构建(项目编号:2022A03004-1)" （项目编号:2022A03004-1）
国家自然基金"新疆野生黄花苜蓿种质资源遗传特性与核心种质构建研究(项目编号:31860675)" （项目编号:31860675）
