汕头大学医学院学报2024，Vol.37Issue(1)：7-11,5.DOI:10.13401/j.cnki.jsumc.2024.01.002
白藜芦醇对糖尿病心脏微血管损伤的作用及机制研究
The effect and mechanism of resveratrol on diabetic cardiac microvascular injury
蔡思琳 1汪彬2
作者信息
- 1. 汕头大学医学院药理学教研室,广东 汕头 515041||汕头市中心医院药学部,广东 汕头 515041
- 2. 汕头大学医学院药理学教研室,广东 汕头 515041
- 折叠
摘要
Abstract
Objective:To investigate the effect and mechanism of resveratrol on diabetic cardiac microvascular injury.Methods:Rat cardiac microvascular endothelial cells were isolated and cultured,and randomly divided into normal glucose group,mannitol group,high glucose group,high glucose + resveratrol group and high glucose + resveratrol + compound C group.The cellular reactive oxygen species level was detected by flow cytometry,superoxide dismutase activity and malondialdehyde content were detected by commercial assay kit,and the activity of AMP-activated protein kinase(AMPK)was detected by Western blot.Results:Compared with the normal group,reactive oxygen species level and malondialdehyde content were increased,and superoxide dismutase activity and p-AMPK/AMPK value were decreased in the high glucose group.Compared with the high glucose group,reactive oxygen species level and malondialdehyde content were decreased,and superoxide dismutase activity and p-AMPK/AMPK value were increased in the high glucose + resveratrol group.Compared with the high glucose + resveratrol group,reactive oxygen species levels and malondialdehyde content were increased and superoxide dismutase activity and p-AMPK/AMPK values were decreased in the high glucose + resveratrol + compound C(AMPK inhibitor)group,and the differences were statistically significant(all P<0.05).Conclusion:Resveratrol attenuates diabetic cardiac microvascular injury by activating AMPK.关键词
白藜芦醇/糖尿病/心脏微血管/损伤Key words
resveratrol/diabetes mellitus/cardiac microvascular/injury分类
医药卫生引用本文复制引用
蔡思琳,汪彬..白藜芦醇对糖尿病心脏微血管损伤的作用及机制研究[J].汕头大学医学院学报,2024,37(1):7-11,5.
