山东农业科学2024，Vol.56Issue(3)：11-18,8.DOI:10.14083/j.issn.1001-4942.2024.03.002
石榴蔗糖代谢相关酶SPS和INV基因家族鉴定与表达分析
Identification and Expression Analysis of Sucrose Phosphate Synthase(SPS)and Invertase(INV)Gene Families in Pomegranate
摘要
Abstract
Sucrose phosphate synthetase(SPS)and sucrose invertase(INV)are key regulatory enzymes of sucrose metabolism and play important roles in plant growth and development.Bioinformatics and fluores-cence quantitative PCR were used to identify the SPS and INV gene family members of pomegranate and ana-lyze their physical and chemical properties,conserved domains,conserved motifs,secondary structure,sub-cellular localization,phylogenetic relationships and expression patterns in the study.The results showed that 4 SPS genes and 11 INV genes were identified from pomegranate,whose encoded proteins were all unstable ones with typical conserved domains.The number and types of motifs among the family members were roughly the same,indicating highly conserved protein structures.These members were distributed unevenly on chromo-somes and located in chloroplasts.The secondary structure of these proteins mainly consisted of α-helices and random curls.The SPS and INV gene family members of pomegranate had different degrees of relationship,and had higher homology with the gene family members of Eucalyptus grandis.The expression patterns of SPS and INV gene family members in pomegranate were different at different fruit developmental stages.The relative ex-pression level of PgINV3 was the highest on September 15(fruit enlargement stage),which was significantly higher compared with the other periods.The study results had great significance to elucidating the molecular mechanism of sucrose metabolism in pomegranate fruits.关键词
石榴/SPS基因/INV基因/生物信息学分析/基因表达Key words
Punica granatum L./SPS gene/INV gene/Biological information analysis/Gene expression分类
农业科技引用本文复制引用
冯立娟,李英朋,王传增,尹燕雷,郭琳,谭伟..石榴蔗糖代谢相关酶SPS和INV基因家族鉴定与表达分析[J].山东农业科学,2024,56(3):11-18,8.基金项目
枣庄学院山东省石榴精深加工工程技术研究中心/山东省石榴资源综合开发工程实验室开放课题(SLKF2021001) （SLKF2021001）
山东省重点研发计划项目(2022TZXD009) （2022TZXD009）
山东省农业科学院农业科技创新工程项目(CXGC2023A12) （CXGC2023A12）
山东省农业科学院揭榜科技难题项目(SHJB2022-42) （SHJB2022-42）
