山东农业科学2024，Vol.56Issue(3)：19-31,13.DOI:10.14083/j.issn.1001-4942.2024.03.003
春大白菜可溶性糖代谢及转运相关基因表达模式分析
Expression Patterns of Soluble Sugar Metabolism and Transport Related Genes of Spring Chinese Cabbage
摘要
Abstract
In this research,spring Chinese cabbage'Jijin'and'Huanai'were used to analyze the changes of soluble sugars and the expression patterns of related metabolic enzyme and transport protein genes during the entire development process.The results showed that during the development process of spring Chi-nese cabbage,total soluble sugar and glucose accumulated rapidly during the rosette stage and early heading stage,and glucose accumulation was dominant;fructose content rapidly increased in the midrib of the leafy head during the late period of the heading stage.The midrib of the rosette leaves and heading leaves were im-portant parts for accumulation of soluble sugars.Sucrose synthase gene BrSUS1 was closely related to soluble sugar accumulation in midrib of the two cultivars.The enzyme genes BrSUS5,BrSUS6,BrCNIV2 decomposing sucrose,as well as the sugar transport protein genes BrSUC4,BrTMT1,BrSWEET17 and BrERDL6-16,were actively expressed in the midrib,which participating to varying degrees in accumulation and distribution of sol-uble sugars in the two Chinese cabbage varieties.The expression level of BrERDL6-16 sharply increased in several tissues of the two cultivars at late heading stage,which might play a special role in promoting the de-velopment of Chinese cabbage leafy head.The results of this study could provide theoretical bases for regula-ting the accumulation and distribution of soluble sugars and the yield formation of Chinese cabbage at molecu-lar level.关键词
春大白菜/可溶性糖代谢与转运/基因表达Key words
Spring Chinese cabbage/Soluble sugar metabolism and transport/Gene expression分类
农业科技引用本文复制引用
陈熙,程斐,倪润茂,刘维信,刘倩倩..春大白菜可溶性糖代谢及转运相关基因表达模式分析[J].山东农业科学,2024,56(3):19-31,13.基金项目
中央引导地方科技发展专项计划项目(23-1-3-5-zyyd-nsh) （23-1-3-5-zyyd-nsh）
山东省重点研发计划项目(2019GNC106009) （2019GNC106009）
山东省农业良种工程项目(2019LZGC006) （2019LZGC006）
