汕头大学医学院学报2024，Vol.37Issue(1)：35-38,4.DOI:10.13401/j.cnki.jsumc.2024.01.008
细菌16S rRNA基因检测在新生儿败血症诊断中的价值
The value of bacterial 16S rRNA gene testing in the diagnosis of neonatal sepsis
摘要
Abstract
Objective:To investigate the value of bacterial 16S rRNA gene testing in the diagnosis of neonatal sepsis.Methods:102 suspected septic neonates admitted to the First Affiliated Hospital of Shantou University Medical College from December 2016 to January 2018 were selected for the study,including 66 males and 36 females aged 10 min-28 d.Blood specimens from the study subjects were collected for pathogen detection by blood culture and bacterial 16S rRNA gene PCR assay,respectively,and blood specimens from 10 neonates hospitalized for non-infectious diseases during the same period were selected as controls.Results:Forty-six out of 102 suspected septic neonates were diagnosed with sepsis by clinical diagnosis and laboratory confirmation,the positive rate of blood culture was 32.61%(15/46),the positive rate of 16S rRNA gene test was 91.30%(42/46),and the positive rate of 16S rRNA gene test was higher than that of blood culture(P<0.001).The results of PCR test and blood culture of 10 children with non-infectious diseases hospitalized in the same period were negative,and the sensitivity of PCR test was 91.30%(42/46),and the specificity was 96.43%(54/56).Conclusion:The bacterial 16S rRNA gene test has a high positive rate and is sensitive and accurate compared to traditional blood cultures.关键词
新生儿败血症/16S rRNA基因/血培养Key words
neonatal sepsis/16S rRNA gene/blood culture分类
医药卫生引用本文复制引用
林丹娥,翁立坚,杨斯岚,林霓阳,房晓祎..细菌16S rRNA基因检测在新生儿败血症诊断中的价值[J].汕头大学医学院学报,2024,37(1):35-38,4.基金项目
广东省高水平大学重点学科建设项目(2050205) （2050205）
深圳市科技计划项目(JCYJ20190809145409829) （JCYJ20190809145409829）
