山东农业科学2024，Vol.56Issue(3)：132-138,7.DOI:10.14083/j.issn.1001-4942.2024.03.018
一株裂解耐氨苄西林金黄色葡萄球菌的噬菌体及其生物学特性与全基因组分析
Biological Characteristics and Complete Genome Analysis of an Ampicillin-Resistant Staphylococcus aureus Phage
摘要
Abstract
This study aimed to isolate phages with high efficiency for lysing drug-resistant Staphylococcus aureus,and to provide candidate materials for developing phage biological products.In this study,using ampi-cillin-resistant S.aureus as host bacterium,the phage was isolated by double-layer plate method,and then its morphology was observed by transmission electron microscopy,followed by biological characteristics and whole genome sequencing analyses.The results showed that a S.aureus phage with the titer of 2.75×1010 PFU/mL was isolated,named as SP688.The phage had a long uncontracted tail,which belonged to Caudovirales,the long tail phage family.It was capable of lysing 7 strains of ampicillin-resistant S.aureus with the optimal com-plex number of infection as 1;it could inhibit the bacterial growth within 10 hours with stable activity at-20℃to 54℃and pH 3～11;it was sensitive to UV.The length of SP688 whole genome was 45 499 kb,defined as a circular DNA with GC content as 34.1% .A total of 64 ORFs were found,and 19 ones(29.69% )were predicted in function.This phage was a novel broad-spectrum one lysing ampicillin-resistant S.aureus,which might provide research material for phage therapy.关键词
金黄色葡萄球菌/噬菌体/形态/生物学特性分析/全基因组分析Key words
Staphylococcus aureus/Phage/Morphology/Biological characteristics analysis/Whole ge-nome analysis分类
农业科技引用本文复制引用
张雪丽,于浩淼,闫振贵,杨少华,张亮,杨宏军..一株裂解耐氨苄西林金黄色葡萄球菌的噬菌体及其生物学特性与全基因组分析[J].山东农业科学,2024,56(3):132-138,7.基金项目
国家现代农业产业技术体系项目(CARS36) （CARS36）
山东省重点研发计划(乡村振兴科技创新提振行动计划)项目"预制菜全链条智慧生产技术创新与应用"(2022TZXD0021) （乡村振兴科技创新提振行动计划）
山东省农业科学院农业科技创新工程项目(CXGC2022A28) （CXGC2022A28）
