生物技术通报2024，Vol.40Issue(3)：100-108,9.DOI:10.13560/j.cnki.biotech.bull.1985.2023-0540
增加革兰阴性菌外膜囊泡产量的研究进展
Research Progress in Increasing Yield of Outer Membrane Vesicles from Gram Negative Bacteria
摘要
Abstract
Outer membrane vesicles(OMVs)are vesicle-like structures released by gram-negative bacteria,and OMVs play an important role in gram-negative bacteria growth,infection and communication with the environment.Because OMVs are inactive,non-replicable and possess high immunogenicity,OMVs are widely applied for research and development of novel vaccines.However,the natural OMVs yield is too low to meet scale-up production.To increase OMVs yield is the key technical issue that should be resolved for scale-up production.We mainly review on three aspects for increasing OMVs yield,modification on bacterial genes,optimization on bacterial growth conditions and OMVs production and purification,aiming to provide informative reference for industrial research and development of OMVs vaccine.关键词
外膜囊泡/疫苗开发/生物发生机制/产量增加机制Key words
outer membrane vesicles/vaccine development/biogenesis mechanism/yield enhancement mechanism引用本文复制引用
刘灿,闫晓阳,曾焱,欧祥龙,廖永洪..增加革兰阴性菌外膜囊泡产量的研究进展[J].生物技术通报,2024,40(3):100-108,9.基金项目
国家重点研发计划项目(2022YFD1800902),中央高校基本科研业务费-人才引进项目(SWU020020),国家生猪技术创新中心项目(NCTIP-XD/B11 （2022YFD1800902）
NCTIP-XD/C17) （）
