生物技术通报2024，Vol.40Issue(3)：118-134,17.DOI:10.13560/j.cnki.biotech.bull.1985.2023-0857
毕赤酵母中外源蛋白表达量的提升策略
Strategies for Increasing Heterologous Protein Expression in Pichia pastoris
摘要
Abstract
The production of heterologous proteins via recombinant expression system is a focus of genetic engineering research.Because of its ease of genetic manipulation,high degree of heterologous protein secretion,and post-translational modification,Pichia pastoris,a methylotrophic yeast,has become an essential strain for protein production in industrial applications and is frequently employed in the manufacturing of enzymes.Although some heterologous proteins can be expressed in P.pastoris,the amount of expression is modest and has to be increased.Therefore,it is critical to investigate the principle and method of boosting the expression of heterologous proteins in P.Pastoris in order to lower industrial production costs and improve economic benefits.In this review,the optimizing strategies for improving heterologous protein expression in P.Pastoris in recent years are summarized from six perspectives:Gene level,transcription level,translation level,protein folding and secretion levels,cell resistance level,and fermentation technology,with the goal of demonstrating comprehensively how to improve the expression of heterologous protein in P.Pastoris expression system.关键词
毕赤酵母/重组表达/外源蛋白/优化/酶Key words
Pichia pastoris/recombinant expression/heterologous protein/optimization/enzyme引用本文复制引用
茹扎·也里扎提,杨宇..毕赤酵母中外源蛋白表达量的提升策略[J].生物技术通报,2024,40(3):118-134,17.基金项目
青海省重点研发与转化项目(2022-NK-115),国家自然科学基金委员会-欧盟委员会(NSFC-EU)环境生物技术国际合作重点项目(31961133015) （2022-NK-115）
