饲料研究2024，Vol.47Issue(4)：56-62,7.DOI:10.13557/j.cnki.issn1002-2813.2024.04.011
人工养殖对尼罗罗非鱼肠道菌群的影响
Effect of artificial culture on intestinal flora of Nile tilapia
摘要
Abstract
The purpose of the experiment was to study the effect of artificial culture on intestinal flora of Nile tilapia.The intestinal contents of wild(YS)and cultured(YZ)tilapia were sequenced by 16S rDNA sequencing method,and the differences of intestinal flora richness and diversity between them were analyzed and compared.The results showed that Actinobacteriota,Proteobacteria and Firmicutes were the main dominant flora in the intestinal tract of Nile tilapia in wild environment.Actinobacteriota,Proteobacteria and Cyanobacteria were the main dominant flora in artificial culture environment.The analysis of intestinal flora diversity showed that the community diversity index in YZ group was lower than that in YS group(P＜0.05).At the phylum level,Proteobacteria,Acidobacteriota,Bacteroidota and NBl-j in YZ group were lower than those in YS group(P＜0.01 or P＜0.05),while Cyanobacteria and Dependentiae were higher than those in YS group(P＜0.01 or P＜0.05).At the genera level,the genera of norank_f norank_o Chloroplast,Mycobacterium,Paeniclostridium,IMCC26207,Methylocystis and Conexibacter in YZ group were higher than those in YS group(P＜0.0l),while the genera of Exiguobacterium,norank_f_Steroidobacteraceae and norank_f_SC-I-84 were lower than those in YS group(P＜0.0l).Spearman correlation analysis showed that the order of environmental factors on the distribution of species data was pH＞NH4-N＞NO2-N＞O＞PO4-P.At the level of genus classification,the influence of environmental factors on the distribution of species data in different grouping samples was in the order of NO2-N＞NH4-N＞pH＞O＞PO4-P.The study indicates that artificial culture will change the intestinal flora of Nile tilapia,which is helpful to explain the effects of intestinal flora on the health and growth of Nile tilapia in cultured and wild environments.关键词
尼罗罗非鱼/肠道菌群/不同养殖环境/16SrDNA序列Key words
Nile tilapia/intestinal flora/different culture environments/16S rDNA sequence分类
农业科技引用本文复制引用
周玥祺,黄杰,王志强,邓小红,林举星,吴芸露,易弋..人工养殖对尼罗罗非鱼肠道菌群的影响[J].饲料研究,2024,47(4):56-62,7.基金项目
广西创新驱动发展专项(项目编号:AA20302019-5) （项目编号:AA20302019-5）
柳州市科技计划项目(项目编号:2020NACB0802) （项目编号:2020NACB0802）
