生物技术通报2024，Vol.40Issue(3)：135-145,11.DOI:10.13560/j.cnki.biotech.bull.1985.2023-0862
TMT定量蛋白质组学解析Rummeliibacillus suwonensis 3B-1生长及己酸代谢机制
Analyzing the Growth and Caproic Acid Metabolism Mechanism of Rummeliibacillus suwonensis 3B-1 by Tandem Mass Tag-based Quantitative Proteomics
摘要
Abstract
[Objective]This work aims to elucidate the growth and caproic acid metabolism mechanism of Rummeliibacillus suwonensis from protein level,and to further improve its metabolic capacity.It provides a technical basis for the genetic engineering of R.suwonensis.[Method]The differential expression proteins(DEPs)of strain R.suwonensis 3B-1 were extracted by tandem mass tags(TMT)proteomics in aerobic and anaerobic conditions.Its subcellular localization analysis,Gene Ontology(GO)functional analysis,KEGG signaling pathway annotation analysis,and protein-protein interaction were analyzed.[Result]A total of 810 DEPs were identified,including 423 up-regulated proteins and 387 down-regulated proteins.A total of 725 DEPs were subcellular mapped to 6 items,mainly involving cytoplasmic proteins,cytoplasmic membrane proteins and cellwall proteins.GO enrichment of function analysis indicated that,biological processes such as peptide biosynthesis,translation,and peptide metabolism,molecular functions such as structural constituent and structural molecular activity of ribosomes,and cellular components such as ribosomes and ribonucleoprotein complexes have undergone significant changes.A total of 810 DEPs were annotated into 113 KEGG signaling pathways in KEGG database,which mainly were involved cofactor biosynthesis,two-component system,pentose phosphate pathway,glycolysis/gluconeogenesis and oxidative phosphorylation.The phenylalanine-tRNA ligase β subunit and the riboflavin biosynthesis protein RibD had the highest correlation in the protein interaction network.[Conclusion]Under anaerobic conditions,the expression of pyruvate dehydrogenase and pyruvate kinase in the glycolytic pathway was down-regulated.Additionally,proteins related to amino acid metabolism and biotin protein ligase cofactors were also downregulated.This indicates that the bacterium is better suited for growth in an aerobic conditions.Regarding the synthesis of caproic acid,the expression of acyl-CoA thioesterase was significantly up-regulated.In addition,the glycolysis/gluconeogenesis pathway,the tricarboxylic acid cycle and the pentose phosphate pathway provided sufficient precursors and reduction equivalents for caproic acid synthesis,which jointly promoted caproic acid synthesis.关键词
TMT蛋白质组学/Rummeliibacillus suwonensis/己酸/生物信息学Key words
TMT proteomics/Rummeliibacillus suwonensis/caproic acid/bioinformatics引用本文复制引用
陈晓松,刘超杰,郑佳,乔宗伟,罗惠波,邹伟..TMT定量蛋白质组学解析Rummeliibacillus suwonensis 3B-1生长及己酸代谢机制[J].生物技术通报,2024,40(3):135-145,11.基金项目
中国轻工业浓香型白酒固态发酵重点实验室开放基金项目(2021JJ017) （2021JJ017）
