生物技术通报2024，Vol.40Issue(3)：181-192,12.DOI:10.13560/j.cnki.biotech.bull.1985.2023-0960
马铃薯StDof5的克隆及表达分析
Cloning and Expression Analysis of StDof5 Gene in Potato
摘要
Abstract
[Objective]The DNA binding with one finger(Dof)gene family is a plant-specific transcription factor family that plays important roles in plant growth,development and stress response.Studying the function and mechanism of Dof transcription factors responding to abiotic stress,especially salt stress in potato,provides some theoretical and technical basis for obtaining new varieties resistant to salt stress.[Method]StDof5 was cloned,and it was analyzed via bioinformatics.StDof5 overexpression vector was constructed by homologous recombination,potato was transformed to obtain positive plants lines,and their expressions were analyzed.[Result]CDS of StDof5 is 498 bp,it encodes 165 amino acids,and the molecular formula is C800H1236N242O246S9.The number of StDof5 atoms is 2 533,the relative molecular weight is 18 468.63 kD,the isoelectric point is 8.47,the aliphatic index is 51.45,and the instability coefficient is 45.16,the protein is predicted to be an unstable protein.The predicted number of StDof5 transmembrane helices is 0,thus it is a non-transmembrane protein.The average value of hydrophilicity of StDof5 protein is-0.876,predicting that it is hydrophilic.The predicted secondary structure of StDof5 protein showed it has 20%α-helices,0 β-turn,9.7%extended strands and 66.06%random coils.Salt stress phenotype experiments showed that the plant heights and fresh weights of overexpressing plants(OPs)were significantly higher than those of wild type(DES)plants,but there was no significant difference in root length,root number and leaf number between them.[Conclusion]Overexpressing plants(OPs)can resist salt stress and demonstrate desensitization phenotype to salt stress.StDof5 has significant responses to salt stress and tissue specificity.关键词
马铃薯/StDof5/载体构建/遗传转化Key words
potato/StDof5 gene/vector construction/genetic transformation引用本文复制引用
梅显军,宋慧洋,李京昊,梅超,宋倩娜,冯瑞云,陈喜明..马铃薯StDof5的克隆及表达分析[J].生物技术通报,2024,40(3):181-192,12.基金项目
山西农业大学杂粮种质创新与分子育种国家实验室(筹)(202204010910001),山西省博士毕业生来晋工作奖励科研项目(SXBYKY2021084),山西农业大学博士基金(2021BQ44),山西农业大学生物育种工程(YZGC069),山西省农业科学院作物科学研究所博士基金(ZB1102) （筹）
