生物技术通报2024，Vol.40Issue(3)：229-241,13.DOI:10.13560/j.cnki.biotech.bull.1985.2023-0839
铁皮石斛14-3-3基因家族鉴定及表达分析
Identification and Expression Analysis of 14-3-3 Gene Family in Dendrobium officinale
摘要
Abstract
[Objective]14-3-3 protein,also known as general regulatory factor(GRF),encodes through multi-gene family,and plays a central role in encouraging plant growth and development and stress-related adversity responses.The identification of GRF gene family in Dendrobium officinale may provide theoretical basis for the study of D.officinale GRF gene function and genetic improvement.[Method]Bioinformatics was used to identify the members of 14-3-3 gene family,and analyze their physicochemical properties,chromosomal localization,phylogenetic tree,gene structure,and cis-acting elements of each DoGRF member.Simultaneously,their expression profiles in different tissues and under the treatment of low-temperature or salt stress were detected using the real-time quantitative PCR.[Result]Results demonstrated that seventeen DoGRF gene family members were presented in D.officinale,which harbored two subfamilies(ε group and non-ε group).They were unevenly located on 7 chromosomes,and harbored seven tandem repeat gene pairs.The gene structure,conserved motifs,and secondary structure of DoGRF members within the same subfamily were similar.The cis-acting elements at the promoter regions of DoGRF family genes contained abundant hormone and stress response elements.DoGRF genes family were expressed in various tissues(flower buds,sepals,labellum(lip),pollinia,gynostemium(column),stems,leaves,roots,green root tips,and white part of roots),and most of them were highly expressed in floral organs,followed by stems and roots.Besides,they were differentially expressed under treatment of low-temperature or salt-stress,suggesting that they might be involved in regulating the stresses of low-temperature and salinity.Especially,DoGRF2 might play a crucial role in the process of stress responses.[Conclusion]17 members of DoGRF family were systematically identified from D.officinale at the genome level.Different genes possessed different responses to abiotic stresses,and exhibited tissue-specific expression profiles.DoGRF2 showed positively response to low-temperature and salt stress..关键词
铁皮石斛/14-3-3家族/基因表达/非生物胁迫Key words
Dendrobium officinale/14-3-3 family/gene expression/abiotic stress引用本文复制引用
江林琪,赵佳莹,郑飞雄,姚馨怡,李效贤,俞振明..铁皮石斛14-3-3基因家族鉴定及表达分析[J].生物技术通报,2024,40(3):229-241,13.基金项目
国家自然科学基金项目(32000257),浙江省自然科学基金项目(LY23H280003),浙江省大学生新苗人才计划(2023R410041,2023R410044),广东省自然科学基金项目(2020A1515410004) （32000257）
