中国临床药理学杂志2024，Vol.40Issue(6)：849-853,5.DOI:10.13699/j.cnki.1001-6821.2024.06.014
红芪多糖对db/db小鼠糖尿病心肌病NF-κB/IKKβ信号通路的影响
Effects of Hedysarum polybotrys polysacchcaide on NF-κB/IKKβ signaling pathway in db/db mice with diabetic cardiomyopathy
摘要
Abstract
Objective To investigate the effect of hedysarum polysacchcaide(HPS)on nuclear transcription factor-κB(NF-κB)/IκB kinase β(IKKβ)signaling pathway in cardiac tissue of db/db mice with diabetic cardiomyopathy(DCM).Methods Altogether 60 7-week-old male db/db mice were randomly divided into model group,control group and experimental-H,-M,-L groups,with 12 mice in each group.In addition,12 db/m mice of the same week age were selected as the normal group.Normal group and model group were given 0.9％NaCl by intragastric administration.Experimental-L,-M,-H groups were given 50,100 and 200 mg·kg-1 HPS suspension by intragastriction,respectively.Control group was given 4 mg·kg-1 rosiglitazone suspension by intragastric administration.Six groups of rats were given the drug once a day for 8 weeks.The contents of tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6)in myocardial tissue were detected by enzyme-linked immunosorbent assay.The mRNA expression levels of NF-κB and IKKβ in myocardial tissue were detected by real-time fluorescence quantitative polymerase chain reaction.The correlation between the expression of NF-κB protein and the content of TNF-α and IL-6 was analyzed.Results The contents of IL-6 in myocardial tissue of normal,model,control and experimental-H groups were(1.24±0.54),(7.72±0.24),(2.90±0.50)and(2.78±0.56)ng·L-1;the contents of TNF-α were(1.96±0.52),(5.25±0.72),(2.84±0.86)and(2.82±0.58)ng·L-1;the mRNA expression levels of NF-κB were I.00±0.00,3.35±0.81,2.05±0.44 and 1.67±0.22;the mRNA expression levels of IKKβ were 1.00±0.00,2.92±0.07,1.51±0.07 and 1.41±0.08,respectively.Compared with the model group,the above indexes of the control and experimental-H groups were statistically significant(P＜0.01,P＜0.05).The expression of NF-κB protein was positively correlated with the content of IL-6 and TNF-α,and the correlation coefficients were 0.866 and 0.740(all P＜0.01).Conclusion HPS can alleviate the damage of myocardial pathology in mice,reduce myocardial collagen deposition and fibrosis,its mechanism may be through regulating the expression of NF-κB/IKKβ signaling pathway to play a role in inhibiting the inflammatory reaction.关键词
红芪多糖/糖尿病心肌病/db/db小鼠/核转录因子-κB信号通路/IκB激酶β/病理形态Key words
Hedysarum polybotrys polysacchcaide/diabetic cardiomyopathy/db/db mice/nuclear transcription factor-κB signaling pathway/IκB kinase β/pathological morphology分类
医药卫生引用本文复制引用
张花治,金智生,孙金宁,邵晶,罗向霞..红芪多糖对db/db小鼠糖尿病心肌病NF-κB/IKKβ信号通路的影响[J].中国临床药理学杂志,2024,40(6):849-853,5.基金项目
国家自然科学基金地区科学基金资助项目(81360538) （81360538）
甘肃省高等学校创新基金资助项目(2020A-082) （2020A-082）
甘肃中医药大学中医学一级学科"岐黄英才"导师专项基金资助项目(ZYXKSD-202208) （ZYXKSD-202208）
