中国临床药理学杂志2024，Vol.40Issue(6)：859-863,5.DOI:10.13699/j.cnki.1001-6821.2024.06.016
大黄素调节HIF-1α/VEGF通路对糖尿病大血管病变大鼠血管内皮细胞损伤的影响
Effects of emodin modulation of the HIF-1α/VEGF pathway on vascular endothelial cells damage in diabetic macroangiopathy rats
摘要
Abstract
Objective To investigate the impact of emodin(EM)on vascular endothelial cell injury in rats with diabetes macroangiopathy by regulating hypoxia inducible factor-1α(HIF-1α)/vascular endothelial growth factor(VEGF)signaling pathway.Methods SD rats were divided into blank group and modeling group,the rats in the modeling group were fed with high fat and high sugar combined with N-nitro-L-arginine methyl ester to build the diabetes macroangiopathy model,and the blank group was fed with ordinary diet.The vascular endothelial cells successfully isolated from the thoracic aorta of rats in blank group and modeling group were named control group and model group,respectively.The vascular endothelial cells in the modeling group were divided into model group,dimethyloxallyl glycine(DMOG)group(10 μmol·L-1DMOG),combined group(80 mg·L-1EM+10 μmol·L-1 DMOG)and experimental-L,-M,-H groups(20,40,80 mg·L-1 EM).The apoptosis of rat vascular endothelial cells was detected by flow cytometry;Western blot was applied to detect the expression of HIF-1αand VEGF proteins in rat vascular endothelial cells.Results The apoptosis rates of vascular endothelial cells in experimental-M,-H groups,DMOG group,combined group,model group and control group were(10.18±0.36)％,(6.28±0.20)％,(24.96±1.18)％,(12.36±0.49)％,(18.76±0.68)％and(4.59±0.26)％;HIF-1α protein levels were 0.96±0.07,0.78±0.06,2.03±0.12,1.05±0.13,1.58±0.12 and 0.69±0.05;VEGF protein levels were 0.59±0.05,0.23±0.02,0.98±0.06,0.63±0.04,0.86±0.07 and 0.11±0.01.The above indexes in the model group were compared with the control,DMOG,experimental-M and experimental-H groups,and the above indexes in the combined group were compared with the experimental-H group,and the differences were statistically significant(all P＜0.05).Conclusion EM may inhibit HIF-1α/VEGF pathway to improve vascular endothelial cell injury in rats with diabetes macroangiopathy.关键词
大黄素/缺氧诱导因子1α/血管内皮生长因子信号通路/糖尿病大血管病变/炎症/氧化应激Key words
emodin/hypoxia inducible factor 1α/vascular endothelial growth factor signal pathway/diabetes macroangiopathy/inflammation/oxidative stress分类
医药卫生引用本文复制引用
朱秋霄,郝慧瑶,刘子渤,高明,张芳,周静,郝志华,张力辉,郝咏梅..大黄素调节HIF-1α/VEGF通路对糖尿病大血管病变大鼠血管内皮细胞损伤的影响[J].中国临床药理学杂志,2024,40(6):859-863,5.基金项目
河北省卫生厅课题基金资助项目(20210516) （20210516）
河北省财政厅课题基金资助项目(361004) （361004）
