中国临床药理学杂志2024，Vol.40Issue(6)：864-868,5.DOI:10.13699/j.cnki.1001-6821.2024.06.017
青蒿琥酯改善哮喘幼鼠气道重塑的作用
Effects of artesunate on improving airway remodeling in asthmatic young rats
摘要
Abstract
Objective To investigate the effect of artesunate on airway remodeling induced by ovalbumin in asthmatic young rats and its mechanism.Methods SD rats were randomly divided into normal group,model group(ovalbumin activation),positive control group(on the basis of model group,injected with 0.2 mg·kg-1 dexamethasone),low-dose group(on the basis of model group,injected with 10 mg·kg-1 artesunate),high-dose group(on the basis of model group,injected with 20 mg·kg-1 artesunate)and agonist group(on the basis of model group,injected with 20 mg·kg-1 artesunate and 100 mg·kg-1 nigerin sodium salt),with 10 rats in each group.Airway remodeling related indexes were detected after treatment.Western blot and real-time fluorescence quantitative polymerase chain reaction were used to detect Nod-like receptor protein 3(NLRP3)pathway-related protein and mRNA expression;enzyme-linked immunosorbent assay were used to detect inflammatory factors expression level,and the classification and count of inflammatory cells in alveolar lavage fluid were detected by Wright's-Giemsa Staining.Results The mRNA expression levels of NLRP3 in normal group,model group,positive control group,high-dose group and agonist group were 1.00±0.10,2.36±0.26,1.08±0.11,1.33±0.12,2.14±0.26,respectively;cysteine aspartate proteinase 1(caspase-1)mRNA expression levels were 1.00±0.13,1.92±0.22,1.22±0.12,1.44±0.10,1.82±0.14,respectively;interleukin-17(IL-17)inflammatory factor expression quantity were(22.41±1.15),(56.74±6.54),(28.72±2.75),(32.69±3.73),(58.40±4.46)pg·mL-1;neutrophil count were(4.04±0.32)×106,(12.70±1.05)×106,(4.53±0.30)×106,(4.67±0.18)× 106,(10.19±0.58)× 106 cell·mL-1.Compared the model group with the normal group,the positive group,the high dose group compared with the model group,the agonist group compared with the high dose group,the differences of the above indicators were statistically significant(all P＜0.05).Conclusion Artesunate can significantly improve airway remodeling in ovalbumin induced asthmatic pups,which may be achieved by inhibiting the NLRP3/caspase-1/interleukin 1 β(IL-1β)inflammatory pathway.关键词
青蒿琥酯/哮喘/幼鼠/气道重塑/NOD样受体蛋白3炎症小体/半胱氨酸天冬氨酸蛋白酶1/白细胞介素-1β通路Key words
artesunate/asthma/young mice/airway remodeling/Nod-like receptor protein 3 inflammasome/cysteine aspartic proteinase-1/interleukin-1 β pathway分类
医药卫生引用本文复制引用
王丽艳,鲍星星,边俊梅..青蒿琥酯改善哮喘幼鼠气道重塑的作用[J].中国临床药理学杂志,2024,40(6):864-868,5.基金项目
武汉市卫健委中医药科研基金资助项目(WZ22C18) （WZ22C18）
