多烯磷脂酰胆碱对高脂饮食诱导的肥胖小鼠代谢紊乱的影响OA北大核心CSTPCD
Effects of polyene phosphatidylcholine on metabolic disorders of obese mice induced by high fat diet
目的 研究多烯磷脂酰胆碱改善高脂饮食诱导的代谢紊乱和脂肪肝作用机制.方法 将32只C57BL/6小鼠随机分为空白组、对照组、模型组和实验组.空白组每周2次喂食低脂饮食并腹膜内注射10％葡萄糖200 μL;对照组每周2次喂食低脂饮食并腹膜内注射含有多烯磷脂酰胆碱(PPC)20 μg的10％葡萄糖溶液200 μL;模型组每周2次喂食高脂肪饮食并腹膜内注射10％葡萄糖200 μL;实验组每周2次喂食高脂肪饮食并腹膜内注射含有PPC 20 μg的10％葡萄糖溶液200 μL.称取小鼠体质量,用血糖测试条测量血糖、分析胰岛素抵抗;用生化分析血清和肝组织中三酰甘油(TG)、高密度脂蛋白(HDL)和低密度脂蛋白(LDL)、谷草转氨酶(GOT)、谷丙转氨酶(GPT)的水平;用酶联免疫吸附测定(ELISA)法检测肝肿瘤坏死因子-α(TNF-α)、白细胞介素(IL)-6、IL-8炎症因子水平.结果 空白组、对照组、模型组及实验组小鼠血清TG水平分别为(0.15±0.01)、(0.11±0.01)、(0.21±0.01)和(0.12±0.01)mmol·L-1,LDL水平分别为(0.41±0.01)、(0.25±0.01)、(0.71±0.02)和(0.49±0.01)mmol·L-1,HDL 水平 分别为(3.04±0.06)、(3.17±0.07)、(2.03±0.05)和(3.72±0.01)mmol·L-1,GOT 水平分别为(30.30±0.89)、(31.39±1.18)、(43.04±2.82)和(25.64±0.72)mmol·L-1,GPT 水平分别为(9.15±0.45)、(7.39±1.88)、(12.87±1.81)和(7.96±1.64)mmol·L-1,空腹血糖水平分别为(4.97±0.08)、(6.08±0.18)、(8.12±0.20)和(7.29±0.02)mmol·L-1,空腹胰岛素水平分别为(6.52±1.11)、(5.45±0.28)、(54.83±4.32)和(30.55±2.73)mU·L-1,肝组织 TNF-α 水平分别为(3.98±0.63)、(3.95±0.98)、(20.55±4.71)和(15.28±1.73)pg·g-1,IL-6 水平分别为(18.93±8.56)、(17.64±3.29)、(59.40±4.63)和(37.54±7.33)pg·g-1,IL-8水平分别为(67.16±12.37)、(59.44±3.58)、(198.40±9.27)和(132.10±7.04)pg·g-1.实验组上述指标与模型组比较,在统计学上差异均有统计学意义(均P＜0.05).结论 多烯磷脂酰胆碱可能通过介导抑制TNF-α、IL-6、IL-8炎症因子的表达,抑制炎症对肝组织的浸润继而改善代谢紊乱.
Objective To study the mechanism of polyene phosphatidylcholine in improving metabolic disorders and fatty liver induced by high fat diet.Methods Thirty-two C57BL/6 mice were randomly divided into blank group,control group,model group and experimental group.The blank group was fed with low-fat diet and intraperitoneal injection of 10％glucose 200 μL twice a week.Control group was fed with low-fat diet twice a week and intraperitoneally injected 10％glucose solution 200 μL containing polyene phosphatidylcholine(PPC)20 μg.Model group was fed with high-fat diet and intraperitoneal injection of 10％glucose 200 μL twice a week.Experimental group was fed with high-fat diet twice a week and intraperitoneally injected 10％glucose solution 200 μL containing PPC 20 μg.The body weight of the mice was measured,blood glucose test strips and insulin resistance was analyzed.The levels of triglyceride(TG),high density lipoprotein(HDL),low density lipoprotein(LDL),glutamic oxalic aminotransferase(GOT)and glutamic pyruvic aminotransferase(GPT)in serum and liver were analyzed by biochemical method.The levels of tumor necrosis factor-α(TNF-α),interleukin-6 and IL-8 were detected by enzyme-linked immunosorbent assay(ELISA).Results The serum TG levels of blank group,control group,model group and experimental group were(0.15±0.01),(0.11±0.01),(0.21±0.01)and(0.12±0.01)mmol·L-1;LDL levels were(0.41±0.01),(0.25±0.01),(0.71±0.02)and(0.49±0.01)mmol·L-1;GOT levels were(30.30±0.89),(31.39±1.18),(43.04±2.82)and(25.64±0.72)mmol·L-1;GPT levels were(9.15±0.45),(7.39±1.88),(12.87±1.81)and(7.96±1.64)mmol·L-1;fasting blood glucose levels were(4.97±0.08),(6.08±0.18),(8.12±0.20)and(7.29±0.02)mmol·L-1;fasting insulin levels were(6.52±1.11),(5.45±0.28),(54.83±4.32)and(30.55±2.73)mU·L-1;the levels of TNF-α in liver tissues were(3.98±0.63),(3.95±0.98),(20.55±4.71)and(15.28±1.73)pg·g-1;IL-6 levels were(18.93±8.56),(17.64±3.29),(59.40±4.63)and(37.54±7.33)pg·g-1;IL-8 levels were(67.16±12.37),(59.44±3.58),(198.40±9.27)and(132.10±7.04)pg·g-1.The difference of above indicatory between experimental group and model group was statistically significant(all P＜0.05).Conclusion Polyene phosphatidylcholine may inhibit the expression of TNF-α,IL-6 and IL-8 inflammatory factors by mediating the inhibition of inflammation on liver tissue and then improve metabolic disorders.
李偲;卢秉久;齐兆东;郑佳连
辽宁中医药大学第一临床学院,辽宁沈阳 110031辽宁中医药大学附属医院肝病科,辽宁沈阳 110032辽宁中医药大学附属第二医院儿科,辽宁沈阳 110034
药学
多烯磷脂酰胆碱高脂饮食脂肪肝代谢紊乱动物模型炎症因子
polyene phosphatidylcholinehigh-fat dietfatty livermetabolic disordersanimal modelinflammatory factors
《中国临床药理学杂志》 2024 (006)
874-878 / 5
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