中国体外循环杂志2024，Vol.22Issue(1)：44-49,64,7.DOI:10.13498/j.cnki.chin.j.ecc.2024.01.10
岩白菜素对大鼠心肌细胞的缺氧/复氧损伤保护作用的机制研究
Bergenia alleviates simulate ischemia/reperfusion injury of cardiomyocytes in rats via SIRT1/PCG-1 α
摘要
Abstract
Objective To explore the molecular mechanism of Bergenin in alleviating ischemia-reperfusion(SI/R)injury in rat cardiomyocytes(H9C2).Methods H9C2 cells were modeled to simulate SI/R damage,the expression of key molecules was detected by WB,cell damage was detected by TUNEL staining and LDH release,intracellular reactive oxygen species levels were detected by DCFH-DA staining and MDA,and SIRT1 and PGC-1α were inhibited by EX-527 and SR-18292,respectively.The experimental groups were:the intervention group(SI/R+Ber),the intervention group(SI/R+Ber+EX-527),and the control group(SI/R+PBS).Results Compared with the SI/R+PBS group,the expression level of Bcl-2 in the SI/R+Ber group increased significantly(P<0.001),the expression level of Bax decreased significantly(P<0.001),and the expression level of PGC-1α increased significantly(P<0.001).Compared with the SI/R+Ber group,the expression level of Bcl-2 in the SI/R+Ber+Ex-527 group decreased significantly(P<0.001),the expression level of Bax increased significantly(P<0.001),and the expression level of PGC-1α decreased significantly(P<0.001).After administration of the inhibitor SR-18292 of PGC-1α,cell viability was significantly reduced compared with the SI/R+Ber group(P<0.01),LDH release was significantly increased(P<0.001),MDA production was significantly increased(P<0.01),apoptosis index of cardiomyocytes was significantly increased(P<0.01),ROS production was significantly increased(P<0.01),Bcl-2 expression level was significantly decreased(P<0.001),Bax expression level was increased significantly(P<0.01),while SIRT1 expression level was not significantly changed(P>0.05).Conclusion Bergenin activates SIRT1/PGC-1 α pathway to reduce ROS production,and then inhibit myocardial cell apoptosis in myocardial SI/R injury.关键词
岩白菜素/缺氧/复氧损伤/沉默交配型信息调控2同源物1/过氧化物酶体增殖物受体共激活因子-1α/活性氧/氧化应激Key words
Bergenin/Myocardial ischemia-reperfusion injury/Silent mating type information regulation 2 homolog-1/Peroxisome proliferator activate receptorγcoactivator-lα/Reactive oxygen species/Oxidative stress引用本文复制引用
杜艳梅,田展松,谭延振,孙阳..岩白菜素对大鼠心肌细胞的缺氧/复氧损伤保护作用的机制研究[J].中国体外循环杂志,2024,22(1):44-49,64,7.基金项目
国家自然科学基金(82270286) （82270286）
