中国临床药理学杂志2024，Vol.40Issue(6)：830-833,4.DOI:10.13699/j.cnki.1001-6821.2024.06.010
银莲花素A对结肠癌HCT116细胞增殖和凋亡的影响
Effects of raddeanin A on the proliferation and apoptosis of colon cancer HCT116 cells
摘要
Abstract
Objective To investigate the effects of raddeanin A(RA)on the proliferation and apoptosis of HCT116 cells and on the β-catenin/c-Myc pathway.Methods Human colon cancer HCT116 cells were divided into four groups:Control group,experimental-L group,experimental-M group and experimental-H group.Experimental-L,experimental-M,experimental-H groups were treated with 5,10 and 20 μmol·L-1raddeanin A,and the control group was given the same amount of normal saline,respectively.The inhibitory effect of RA on the proliferation of HCT116 cells of colon cancer was detected by cell counting kit-8(CCK-8)method.Cell nucleus morphology change was observed with the fluorescence;the apoptosis rate was detected by flow cytometry;and the expression of related proteins of β-catenin/c-Myc signaling pathway was detected by western blot.Results After 48 h,the cell inhibitory rates of the control group,experimental-L,experimental-M,experimental-H groups were 0,(19.15±0.65)％,(35.11±0.40)％and(49.93±1.13)％,respectively;the cell apoptosis rates were(0.16±0.18)％,(9.26±0.42)％,(17.87±2.54)％and(38.10±2.70)％,respectively;the protein expression levels of β-catenin were 0.74±0.03,0.69±0.01,0.33±0.02 and 0.16±0.04,respectively;the protein expression levels of c-Myc were 0.89±0.01,0.54±0.03,0.29±0.03 and 0.13±0.04,respectively;the protein expression levels of Cyclin D1 were 0.84±0.04,0.66±0.01,0.48±0.06 and 0.21±0.03,respectively;the expression levels of Cleaved-Caspase3 protein were 0.19±0.03,0.26±0.04,0.45±0.04 and 0.78±0.01,respectively.The above indicators in the experimental-L,experimental-M,experimental-H groups showed statistically significant differences compared to those of control group(all P＜0.05).Conclusion RA can inhibit the proliferation of HCT116 cells and induce apoptosis,which may be related to the inhibition of β-catenin/c-Myc signaling pathway.关键词
银莲花素A/β-连环蛋白/结肠癌细胞/增殖/凋亡Key words
raddeanin A/β-catenin/colon cancer/proliferation/apoptosis分类
医药卫生引用本文复制引用
朱雨,姜京植,金雪梅,李莉,朴丽花..银莲花素A对结肠癌HCT116细胞增殖和凋亡的影响[J].中国临床药理学杂志,2024,40(6):830-833,4.基金项目
吉林省科技厅基金资助项目(YDZJ202201ZYTS163) （YDZJ202201ZYTS163）
吉林省卫生健康科技能力提升基金资助项目(2021JC062,2021JC059) （2021JC062,2021JC059）
