广东海洋大学学报2024，Vol.44Issue(2)：32-38,7.DOI:10.3969/j.issn.1673-9159.2024.02.005
低氧胁迫及复氧对香港牡蛎抗氧化和能量代谢相关酶活性的影响
Effects of Hypoxia-reoxygenation on Antioxidant Capacity and Enzyme Activities Related to Energy Metabolism of Crassostrea hongkongensis
摘要
Abstract
[Objective]The aim of this paper is to study the tolerance ability of Crassostrea hongkongensis to hypoxia,and provide data reference for the aquaculture and breeding of hypoxia-tolerant strain of the oysters.[Method]Experiments with 0.5,1.5,and 2.5 mg/L hypoxia stress for 72 h and reoxygenation for 24 h were carried out.The fluctuation of total antioxidant capacity(T-AOC)and enzyme activities related to energy metabolism were measured.[Result]After 6 h of hypoxia stress,the T-AOC,glycogen content,the pyruvate kinase enzyme(PK)and glycolytic phosphofructokinase enzyme(PFK)activity in 0.5 mg/L and 1.5 mg/L hypoxia groups decreased significantly(P<0.05),while the phosphoenolpyruvate carboxykinase(PEPCK)activity has no significant difference compared with the control group(P>0.05).After 72 h,the T-AOC,glycogen content and enzyme activities were increased significantly(P<0.05).After 24 hours of reoxygenation,the metabolism(T-AOC,PK and PFK activities)of oysters in 1.5 mg/L and 2.5 mg/L hypoxia groups were basically restored to normal,no significant difference compared with the control group(P>0.05),while the metabolic level of 0.5 mg/L hypoxia group was still lower than that of the control group(P<0.05).In addition,PEPCK activity in the three hypoxia groups was significantly higher than that of the control group(P<0.05).[Conclusion]Hypoxia significantly affects the antioxidant capacity and enzyme activities related to energy metabolism of Hong Kong oyster.Within 72 h,oysters had better compensatory adaptation to 1.5 and 2.5 mg/L hypoxic stress,but poor compensatory to 0.5 mg/L extreme hypoxic stress.关键词
香港牡蛎/低氧胁迫/复氧/抗氧化能力/能量代谢Key words
Crassostrea hongkongensis/hypoxia stress/reoxygenation/antioxidant capacity/energy metabolism分类
生物科学引用本文复制引用
张兴志,李蔚,李满园,李恒慧,彭金霞,何苹萍,官俊良,韦嫔媛,江林源,张立,陈泳先,罗帮,马元..低氧胁迫及复氧对香港牡蛎抗氧化和能量代谢相关酶活性的影响[J].广东海洋大学学报,2024,44(2):32-38,7.基金项目
国家重点研发计划"海洋农业与淡水渔业科技创新"重点专项(2022YFD2401203,2022YFD2401205) （2022YFD2401203,2022YFD2401205）
广西重点研发项目(桂科AB21196030) （桂科AB21196030）
广西自然科学基金(2023GXNSFBA026311) （2023GXNSFBA026311）
广西水产遗传育种与健康养殖重点实验室自主研究课题(23-A-01-03,22-A-01-01) （23-A-01-03,22-A-01-01）
国家贝类产业技术体系综合试验站项目(CARS-49) （CARS-49）
国家现代农业产业技术体系广西创新团队项目(nycytxgxcxtd-2023-14-05) （nycytxgxcxtd-2023-14-05）
广西农业科技自筹经费项目(Z202290) （Z202290）
