广东海洋大学学报2024，Vol.44Issue(2)：39-45,7.DOI:10.3969/j.issn.1673-9159.2024.02.006
琥珀酰化修饰对马氏珠母贝植核免疫的影响
Effect of Succinylation Modification on Nucleus-implantation Immune Response of Pinctada fucata martensii
摘要
Abstract
[Objective]To study the effect of protein succinylation modification on nucleus-implantation immune response of Pinctada fucata martensii and provide a theoretical basis for elucidation of the molecular regulatory mechanism of nucleus-inserting reaction of pearl oyster.[Method]We simulated increasing the level of succinylation modification by injecting sodium succinate into P.f.martensii,and examined the degree of succinylation of total protein in gill tissues,the expression of immune-related genes,the enzyme activities of antioxidant-related enzymes,as well as the survival and retention rate of pearly shells after nucleus implantation in P.f.martensii.[Result and Conclusion]After injection of sodium succinate,it was found that the level of succinylation in gill tissue increased significantly at 12 h and 72 h.The temporal expression of immune-associated factors was detected using qRT-PCR,and the results showed that the expression of immune-related factors SOD and Caspase2 were significantly up-regulated at 6 h,NFκB and IRAK1 were significantly up-regulated at 48 h,and TRAF3 and IκK mRNA were significantly up-regulated at 96 h(P<0.05),suggesting that cellular immunity of the recipient oysters was enhanced by the increased level of succinylation modification.The enzymatic activity of antioxidant-related enzyme activities SOD,POD and GSH were significantly up-regulated at 96 h(P<0.05),indicating enhanced humoral immunity in the recipient oysters.Compared to the control group(PBS + implantation)and implantation group,the experimental group(sodium succinate+ implantation)'s survival rate was significantly increased at 7 d and 45 d,and the experimental group's retention rate significantly improveed at 60 d(P<0.05).Succinylation modification is involved in the immune response to implantation in P.f.martensii,and injection of sodium succinate improves immune viability of recipient oysters and helps them to survive the difficult post-implantation period.关键词
马氏珠母贝/植核免疫/琥珀酰化修饰/琥珀酸钠Key words
Pinctada fucata martensii/allografting/succinylation modification/sodium succinate分类
农业科技引用本文复制引用
梁海鹰,陈洁,张美珍..琥珀酰化修饰对马氏珠母贝植核免疫的影响[J].广东海洋大学学报,2024,44(2):39-45,7.基金项目
广东省自然科学基金项目(2023A1515012924,2021A1515010962) （2023A1515012924,2021A1515010962）
广东省科技专项资金(2021A05250) （2021A05250）
广东省海港建设与渔业产业发展专项(A201608B15) （A201608B15）
南海经济动物种质创新与利用创新团队项目(2021KCXTD026) （2021KCXTD026）
