广东海洋大学学报2024，Vol.44Issue(2)：62-68,7.DOI:10.3969/j.issn.1673-9159.2024.02.009
盐胁迫对白骨壤种子萌发及幼苗生理的影响
Effects of Salt Stress on Seed Germination and Seedling Physiology of Avicennia marina
摘要
Abstract
[Objective]The aim of this study is to explore the effects of different salt concentrations on the germination of Avicennia marina seeds and the physiology of seedlings.[Method]The experiment was conducted using controlled variable method to investigate the effect of different saline concentrations on the growth of A.marina in sand substrate.The experimental groups were set with artificial seawater salinity levels of 5,10,15,and 20,while distilled water was used as the control group.The plants were cultivated for 120 days,and various growth parameters including root germination rate,development time of true leaves,leaf quantity,plant height,net photosynthetic rate,chlorophyll content,biomass,and stress resistance were measured.[Result]The germination rates of A.marina seeds were(90.3±0.3)%,(94.3±1.2)%,and(91.6±1.5)%in the 0,5,and 10 salinity treatment groups,respectively,which were significantly higher than the salinity 15 treatment group's(75.3±3.5)%and salinity 20 treatment group's(62.3±2.5)%(P<0.05).There was no significant difference in leaf development time and leaf number between the salinity 0,5,and 10 treatment groups(P>0.05).However,in the salinity 15 and 20 treatment groups,the leaf development time was significantly delayed compared to the low salinity treatment group(P<0.05),and the leaf number showed a decreasing trend.The plant height of the groups treated with salinity levels of 0,5,and 10 was significantly higher than the groups treated with 15 and 20(P<0.05).The basal stem diameter of the salinity 5 treatment group was significantly thicker than the salinity 20 treatment group(P<0.05).Among them,the plant height of the salinity 5 treatment group was the highest(33.7±0.8)cm.The total chlorophyll content of the salinity 5 treatment group was significantly higher than the salinity 10,15,and 20 treatment groups,while the salinity 0 and 10 treatment groups were significantly higher than the salinity 15 and 20 treatment groups(P<0.05).The net photosynthetic rate of the salinity 5 treatment group was significantly higher than the other treatment groups,while the salinity 0 and 10 treatment groups were significantly higher than the salinity 15 and 20 treatment groups(P<0.05).Among them,the salinity 5 group had the highest total chlorophyll content and net photosynthetic rate,which were(2.67±0.10)mg/g and(10.32±0.20)μmol·m2·s-1,respectively.The dry mass of leaves,stem,roots,and total biomass of A.marina seedlings in the salinity 5 and 10 groups were significantly higher than those in the salinity 0,15,and 20 groups(P<0.05).Among them,the dry mass of various organs reached the highest value under the salinity 5 condition,significantly higher than other groups(P<0.05).The membrane permeability,malondialdehyde content,proline content,and superoxide dismutase activity in the leaves of A.marina seedlings were significantly higher in the salinity 15 and 20 groups compared to other groups(P<0.05),while these four stress resistance physiological indicators were the lowest in the salinity 5 group,significantly lower than all other groups(P<0.05).[Conclusion]A.marina seeds exhibited high germination rate and good seedling growth under low salinity conditions(0-10),with the most suitable condition observed at salinity 5.However,germination and growth of A.marina were significantly inhibited at salinity levels of 15 and 20.关键词
盐胁迫/白骨壤/红树植物/生长指标/生理指标Key words
salt stress/Avicennia marina/mangrove plants/growth indicators/physiological indicators分类
农业科技引用本文复制引用
梁耐思,曾悦,李党校,黎政杰,林佳峰,段婷婷,李进..盐胁迫对白骨壤种子萌发及幼苗生理的影响[J].广东海洋大学学报,2024,44(2):62-68,7.基金项目
国家自然基金(NSFC32000164) （NSFC32000164）
广东省科学技术厅-2020年省科技专项资金竞争性分配项目(2020A03019) （2020A03019）
2022年"冲补强"省财政专项资金(080503122202) （080503122202）
2022年湛江市科技计划(A23078) （A23078）
