广东海洋大学学报2024，Vol.44Issue(2)：76-86,11.DOI:10.3969/j.issn.1673-9159.2024.02.011
低温与无水胁迫下凡纳滨对虾肌肉品质劣变标记蛋白识别
Identification of Marker Proteins Indicating Potential Flesh Quality Deterioration of Litopenaeus vannamei Under Low Temperature Stress and Waterless Stress
摘要
Abstract
[Objective]The aim of this study is to investigate the correlation between muscle quality deterioration and differently expressed protein in hepatopancreas of Litopenaeus vannamei under low temperature(LT)stress and waterless duration(WD)stress during the anaerobic transportation,and to identify the potentially proteomic markers indicating the quality deterioration.[Method]The physiological responses of shrimp under LT stress and WD stress were observed in a simulated marine environment with a dissolved oxygen content of 7.2 mg/L and a temperature control of(12±1)℃.The significant difference of hepatopancreas proteome induced by the dual stress was analyzed by tandem mass spectrometry,and its correlation with muscle quality was analyzed accordingly.[Results]Compared with the control,33 and 44 differently expressed proteins were identified in LT and LT+WD groups,respectively,among which the most DEPs were significantly enriched in lysosomal,glycolytic or gluconeogenic,and adhesion pathways(P<0.05),while trypsin-1 and dipeptidyl peptidase underwent significant up-regulatied(P<0.05)and phosphoglycan isomerase and aldehyde dehydrogenase were significantly down-regulated(P<0.05).Moreover,the deterioration in colour and texture attributes was closely associated with the expression of tubulin,gelsolin,laminin,endogenous protease and metabolic enzymes.[Conclusion]This research found out that the intrinsic relationship between hepatopancreas DEPs and muscle quality for the first time.The identified typical DEPs could be used as potential marker proteins indicating quality deterioration,and thus providing a molecular target for monitoring muscle quality deterioration of L.vannamei during waterless live transport.关键词
虾/低温暴露/无水保活/品质劣变/蛋白组组学/蛋白标志物Key words
Litopenaeus vannamei/low-temperature exposure/water-free life-preservation/quality deterioration/proteomics/protein markers分类
农业科技引用本文复制引用
徐德峰,廖威龙,郑晓娴,叶盛权,茅林春,谢晶,邓尚贵..低温与无水胁迫下凡纳滨对虾肌肉品质劣变标记蛋白识别[J].广东海洋大学学报,2024,44(2):76-86,11.基金项目
"十三五"国家重点研发计划重点专项(2019YFD0901601) （2019YFD0901601）
广东省自然科学基金(2023A1515010852) （2023A1515010852）
