安徽医科大学学报2024，Vol.59Issue(2)：207-211,5.DOI:10.19405/j.cnki.issn1000-1492.2024.02.004
基于PARP1信号通路研究芒柄花素对糖氧剥夺/复氧复糖神经元细胞损伤的影响
The effect of formononetin on the neuron cell damage of oxygen-glucose deprivation/reoxygenation was studied based on the PARP1 signaling pathway
摘要
Abstract
Objective To study the effect of formononetin on the cell damage of glucose/oxygen deprivation/reoxy-genation glyconeurons via the PARP1 signaling pathway,and to offer theoretical support for the use of Caragana isoflavones in the treatment of cerebral ischemia-reperfusion injury.Methods In mouse neurons(HT22),a model of Oxygen-glucose deprivation/reoxygenation(OGD/R)was created.Western blot was used to detect the expres-sion of PARP1 and PARG in HT22 neurons at various time points of glucose-oxygen deprivation/reoxygenation,and the optimal time point of pathway modification was chosen.After OGD/R,HT22 cells were treated with form-ononetin,PARP1 inhibitor(PJ34),and PARG inhibitor,and six groups were developed:control group,control group+formononetin group,OGD/R group,OGD/R+formononetin group,OGD/R+PJ34 group,OGD/R+PARG inhibitor group.HT22 cells were grown normally without OGD/R therapy in the control group.The expres-sion levels of apoptotic factors and associated proteins in each group were determined using immunofluorescence and Western blot.Results PARP1 pathway was activated most obviously in HT22 cells after 3 hours of glucose and ox-ygen deprivation/reoxygenation.Under the condition of OGD/R 3 h,treatment with formononetin,PJ34 or PARG inhibitor could increase E3 ubiquitin ligase(Iduna),inhibit the expression of PARP1 and PARG pathway proteins,reduce the expression of AIF and P53,and increase the phosphorylation level of AKT protein.Conclusion Form-ononetin can block the PARP1/AIF/Akt signaling pathway by raising the expression of Iduna protein in the pres-ence of OGD/R,hence decreasing the damage to HT22 mouse neurons.关键词
芒柄花素/糖氧剥夺/复氧复糖/神经元损伤/PARP1信号通路Key words
formononetin/OGD/R/neuronal injury/PARP1 signaling pathways分类
医药卫生引用本文复制引用
郁丽,王湄,王文秀,曹丽平,何前松..基于PARP1信号通路研究芒柄花素对糖氧剥夺/复氧复糖神经元细胞损伤的影响[J].安徽医科大学学报,2024,59(2):207-211,5.基金项目
国家自然科学基金(编号:82160850) （编号:82160850）
贵州省科技计划项目(编号:黔科合基础[2020]1Z071) （编号:黔科合基础[2020]1Z071）
