安徽医科大学学报2024，Vol.59Issue(2)：236-242,7.DOI:10.19405/j.cnki.issn1000-1492.2024.02.009
运用孟德尔随机化探索肠道微生物与非酒精性脂肪性肝病的因果关联
Using Mendelian randomization to explore the causal association between gut microbiota and nonalcoholic fatty liver disease
摘要
Abstract
Objective To explore the causal association between gut microbes and non-alcoholic fatty liver disease(NAFLD)by Mendelian randomisation analysis.Methods Genetic instrumental variables for gut microbiota were identified from a gene-wide association study of 18 340 participants,and summary statistics for NAFLD were ob-tained from the FinnGen database,which provided data on 894 NAFLD cases and 217 898 controls using the IVW method as the primary analysis.In order to test the robustness of the results,MR-Egger method,WM method,Simple Mode method,Weighted Mode method were used for Mendelian randomisation analysis,and heterogeneity test,sensitivity analysis,and multiplicity analysis were performed.Results class Gammaproteobacteria IVW re-sults showed(OR=0.621,95％CI=0.412～0.934,P=0.022);family Enterobacteriaceae IVW results showed(OR=1.481,95％CI=1.069～2.053,P=0.018);genus Lachnospiraceae IVW results showed(OR=1.405,95％CI=1.036～1.904,P=0.029);genus Prevotella7 IVW results showed(OR=0.834,95％CI=0.714～0.974,P=0.021);genus Prevotella9 IVW results showed(OR=1.251,95％CI=1.025～1.527,P=0.027);order Desulfovibrionales IVW results showed(OR=0.714,95％CI=0.519～0.982,P=0.038);or-der Enterobacteriales IVW results showed(OR=1.481,95％CI=1.069～2.053,P=0.018).And there was no heterogeneity in the heterogeneity test,and the sensitivity analyses all showed robustness and no pleiotropy was found.Conclusion This study implicates class Gammaproteobacteria,family Enterobacteriaceae,genus Lachno-spiraceae,genus Prevotella7,genus Prevotella9,order Desulfovibrionales,order Enterobacteriales seven species of gut microorganisms have a causal relationship with NAFLD.关键词
肠道微生物/非酒精性脂肪性肝病/孟德尔随机化Key words
gut microbiota/non-alcoholic fatty liver disease/Mendelian randomization分类
医药卫生引用本文复制引用
吴闵,安祯祥,何远利,何松,刘闯,孙凯..运用孟德尔随机化探索肠道微生物与非酒精性脂肪性肝病的因果关联[J].安徽医科大学学报,2024,59(2):236-242,7.基金项目
国家自然科学基金(编号:82160893) （编号:82160893）
国家中医药管理局第五批全国中医临床优秀人才研修项目(编号:国中医药人教函[2022]1号) （编号:国中医药人教函[2022]1号）
贵州中医药大学中医脾胃病科技创新人才团队建设(编号:贵中医TD[2022]005号) （编号:贵中医TD[2022]005号）
