安徽医科大学学报2024，Vol.59Issue(2)：243-248,6.DOI:10.19405/j.cnki.issn1000-1492.2024.02.010
脂多糖干预后的不同滑膜细胞来源炎性外泌体对软骨细胞的作用机制研究
Study on the mechanism of action of different synovial cell-derived inflammatory exosomes on chondrocytes after lipopolysaccharide intervention
摘要
Abstract
Objective To observe the effect of different synovial cell secretions on chondrocytes after LPS-induced inflammation,and to explore the mechanism of two synovial cell secretions causing cartilage damage in the progres-sion of KOA disease.Methods Two kinds of synovial cells were co-cultured at 1∶4 and LPS-induced inflamma-tion.The supernatant and exocrine were extracted,and then the normal and LPS-induced inflammation were extrac-ted.The human cartilage tissue obtained during the operation was isolated and cultured into chondrocytes,which were divided into five groups:the first group was added with FLS secretion,the second group was added with nor-mal FLS secretion,the third group was added with secretion after co-culture of two kinds of synovial cells,the fourth group was added with inflammatory MLS secretion,and the fifth group was added with inflammatory FLS se-cretion.CCK-8 was used to detect the viability of chondrocytes in each group.TNF-α,IL-1β,IL-6 level in the su-pernatant of chondrocytes in each group was detected by ELISA.The protein expression of TLR4,NF-κB,IkK,IκB,ADAMTS5 in chondrocytes of each group was detected by Western blot method.Results CCK-8 showed that the activity of chondrocytes in the three groups of inflammatory secretions decreased compared with the secretions from normal synovial cells(P＜0.05);ELISA showed TNF-α,IL-1 β,IL-6 level in the supernatant of group Ⅲ,Ⅳ and V was higher than that of group Ⅰ and Ⅱ(P＜0.05),TNF-α,IL-1 β,IL-6 level in group Ⅲ was higher than that in group Ⅳ but lower than that in group Ⅴ(P＜0.05).Western blot showed the protein expression of TLR4,NF-κB,IkK,IκB,ADAMTS5 in chondrocytes of group Ⅲ,Ⅳ and Ⅴ was higher than that in group Ⅰ and Ⅱ(P＜0.05),the protein expression of TLR4,NF-κB,IkK,IκB,ADAMTS5 in group Ⅲ was higher than that in group Ⅳbut lower than that in group Ⅴ(P＜0.05).Conclusion Two kinds of synovial cell-derived secretions after LPS-induced inflammation can regulate cartilage TLRs/NF-κB signal pathway,causing cartilage inflammation.The in-flammatory effect of MLS secretion is stronger than that of FLS secretion,but the inflammatory effect of MLS secre-tion under two co-cultures is weaker than that of MLS secretion alone.关键词
成纤维样滑膜细胞/巨噬样滑膜细胞/外泌体/TLRs/NF-κB信号通路/膝骨关节炎/软骨细胞Key words
fibroblast-like synoviocytes/macrophage-like synoviocytes/exosomes/TLRs/NF-κB signal pathway/knee osteoarthritis/chondrocytes分类
医药卫生引用本文复制引用
周俊,郭长青,王庆甫..脂多糖干预后的不同滑膜细胞来源炎性外泌体对软骨细胞的作用机制研究[J].安徽医科大学学报,2024,59(2):243-248,6.基金项目
国家自然科学基金面上项目(编号:81874475) （编号:81874475）
