脂多糖干预后的不同滑膜细胞来源炎性外泌体对软骨细胞的作用机制研究OACSTPCD
Study on the mechanism of action of different synovial cell-derived inflammatory exosomes on chondrocytes after lipopolysaccharide intervention
目的 观察脂多糖(LPS)诱导炎症后的不同滑膜细胞来源外泌体对软骨细胞的影响,探讨两种滑膜细胞外泌体在膝骨关节炎(KOA)疾病进展中引起软骨损伤的作用机制.方法 将两种滑膜细胞以1∶4共培养并用LPS诱导炎症,提取上清液中外泌体,再分别提取正常及LPS诱导炎症后的两种滑膜细胞外泌体.将术中取得的人软骨组织分离培养成软骨细胞,分为五组:第Ⅰ组加入FLS外泌体;第Ⅱ组加入正常的FLS外泌体;第Ⅲ组加入两种滑膜细胞共培养后的外泌体;第Ⅳ组加入炎性的ML…查看全部>>
Objective To observe the effect of different synovial cell secretions on chondrocytes after LPS-induced inflammation,and to explore the mechanism of two synovial cell secretions causing cartilage damage in the progres-sion of KOA disease.Methods Two kinds of synovial cells were co-cultured at 1∶4 and LPS-induced inflamma-tion.The supernatant and exocrine were extracted,and then the normal and LPS-induced inflammation were extrac-ted.The human cartilage tissu…查看全部>>
周俊;郭长青;王庆甫
北京中医药大学第三附属医院骨伤科,北京 100029北京中医药大学针灸推拿学院,北京 100029北京中医药大学第三附属医院骨伤科,北京 100029
临床医学
成纤维样滑膜细胞巨噬样滑膜细胞外泌体TLRs/NF-κB信号通路膝骨关节炎软骨细胞
fibroblast-like synoviocytesmacrophage-like synoviocytesexosomesTLRs/NF-κB signal pathwayknee osteoarthritischondrocytes
《安徽医科大学学报》 2024 (2)
243-248,6
国家自然科学基金面上项目(编号:81874475)
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